Engineering of porous bacterial cellulose toward human fibroblasts ingrowth for tissue engineering
- PDF / 800,186 Bytes
- 12 Pages / 584.957 x 782.986 pts Page_size
- 69 Downloads / 190 Views
Jeffrey M. Catchmark Department of Agricultural and Biological Engineering and Center for Nanocellulosics, Pennsylvania State University, University Park, Pennsylvania 16802, USA
Yongjun Zhu Center for Human Tissue and Organs Degeneration and Shenzhen Key Laboratory of Marine Biomedical Materials, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong 518055, China
Noureddine Abidi Fiber and Biopolymer Research Institute, Department of Plant and Soil Science, Texas Tech University, Lubbock, Texas 79403, USA
Xin Zhou, Jinhui Wang, and Nuanyi Liang Center for Human Tissue and Organs Degeneration and Shenzhen Key Laboratory of Marine Biomedical Materials, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong 518055, China (Received 21 April 2014; accepted 9 October 2014)
From the view of tissue engineering, the deficiency in porosity has impeded further application of bacterial cellulose (BC) as a super biomaterial. In this study, we used a combination method consisting of acetic acid treatment and freeze-drying operation to improve the porous profile of BC, as well as a simple and fast method to measure the thickness, density, and porosity of BC. Results have shown a significant improvement in the porosity of the inner structure of BC treated with acetic acid and freeze-drying. Microscopic observation by scanning electron microscopy exhibited explicit evidences that more orderly porous layer-by-layer structures and more pores were formed along the cross section of modified BC as compared with the control. The enhancement of mechanical properties and crystallinity of modified BC was also demonstrated due to the improvement of material porosity in the particular extent from 50.3 to 76.43%. Cell culture of human fibroblast cells exhibited good cell viability on modified BC, suggesting that a better porous profile of BC on the surface and cross section helps facilitate cells to attach, as well as potentially promotes cells to grow in. These significant results may open the possibility of producing BC nanomaterials for tissue engineering with desirable properties.
I. INTRODUCTION
Bacterial cellulose (BC) is a gelatinous membrane biosynthesized by Gluconacetobacter xylinus at the air–liquid interface in a medium.1 It was first reported by Brown in 1886 and then extensively studied in many studies during the period 1950–1980. 2–5 Nowadays, BC has become one of the promising materials applied in paper, textile, food, and health care industries due to a)
Address all correspondence to this author. e-mail: [email protected] DOI: 10.1557/jmr.2014.315 2682
J. Mater. Res., Vol. 29, No. 22, Nov 28, 2014
http://journals.cambridge.org
Downloaded: 21 Nov 2014
its high chemical purity and ultrafine reticulated structure.6–8 As an alternative material in the biomedical field, BC owns many advantages, including good biocompatibility, excellent fluid exchange ability, various phy
Data Loading...
