Enhanced antioxidant capacity and upregulated transporter genes contribute to the UV-B-induced increase in blinin in Con
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RESEARCH ARTICLE
Enhanced antioxidant capacity and upregulated transporter genes contribute to the UV-B-induced increase in blinin in Conyza blinii Junyi Zhan 1 & Qin Yang 1 & Zhiyi Lin 1 & Tianrun Zheng 1 & Maojia Wang 1 & Wenjun Sun 1 & Tongliang Bu 1 & Zizhong Tang 1 & Chenglei Li 1 & Xueyi Han 1 & Haixia Zhao 1 & Qi Wu 1 & Zhi Shan 1 & Hui Chen 1 Received: 11 July 2020 / Accepted: 2 November 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Conyza blinii (C. blinii) is a traditional Chinese medicinal plant mainly grown in Sichuan, China. C. blinii is suitable for studying the mechanism of plant tolerance to UV-B due to its living conditions, characterized by a high altitude and exposure to strong ultraviolet radiation. Our results showed that the growth and photosynthetic activity of C. blinii were improved under a specific intensity of UV-B, rather than being significantly inhibited. Although UV-B increased the content of reactive oxygen species (ROS) in C. blinii, the activities of antioxidative enzymes were elevated, including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), which contributed to the elimination of ROS. Additionally, the content of blinin, the characteristic diterpene in C. blinii, was markedly increased by UV-B. Furthermore, RNA sequencing analyses were used to explore the molecular mechanism of UV-B tolerance in C. blinii. According to the results, most of the key enzyme genes in the blinin synthesis pathway were upregulated by UV-B. In addition, 23 upregulated terpene transporter genes were identified, and these genes might participate in blinin transport during the response to UV-B. Taken together, these results implied that enhanced antioxidant capacity and upregulated transporter genes contributed to increased synthesis of blinin in response to UV-B in C. blinii. Keywords Conyza blinii . UV-B . Blinin . RNA sequencing . Transporter . Antioxidant capacity
Junyi Zhan and Qin Yang contributed equally to this work. Responsible Editor: Gangrong Shi * Hui Chen [email protected]
Zizhong Tang [email protected]
Junyi Zhan [email protected]
Chenglei Li [email protected]
Qin Yang [email protected]
Xueyi Han [email protected]
Zhiyi Lin [email protected]
Haixia Zhao [email protected]
Tianrun Zheng [email protected]
Qi Wu [email protected]
Maojia Wang [email protected]
Zhi Shan [email protected]
Wenjun Sun [email protected] Tongliang Bu [email protected]
1
College of Life Science, Sichuan Agricultural University, Ya’an, China
Environ Sci Pollut Res
Introduction Ultraviolet-B (UV-B) stress is a widely studied abiotic stress (Rozema et al. 1997) that can lead to changes in plant growth (Prado et al. 2012), physiology, biochemistry (Zhang et al. 2019), photosystems (Teramura et al. 2010), and antioxidant activity (Agarwal 2007), as well as damage or changes of biofilms (Kramer et al. 1991), DNA (Takeuchi et al. 1996), proteins (Campbell et al. 1998), and plant hormones (Jenkins and Brown 2
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