Major latex protein-like encoding genes contribute to Rhizoctonia solani defense responses in sugar beet
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ORIGINAL ARTICLE
Major latex protein‑like encoding genes contribute to Rhizoctonia solani defense responses in sugar beet Louise Holmquist1,2 · Fredrik Dölfors2 · Johan Fogelqvist2 · Jonathan Cohn3 · Thomas Kraft1 · Christina Dixelius2 Received: 2 June 2020 / Accepted: 9 October 2020 © The Author(s) 2020
Abstract Sugar beets are attacked by several pathogens that cause root damages. Rhizoctonia (Greek for “root killer”) is one of them. Rhizoctonia root rot has become an increasing problem for sugar beet production and to decrease yield losses agronomical measures are adopted. Here, two partially resistant and two susceptible sugar beet genotypes were used for transcriptome analysis to discover new defense genes to this fungal disease, information to be implemented in molecular resistance breeding. Among 217 transcripts with increased expression at 2 days post-infection (dpi), three resistance-like genes were found. These genes were not significantly elevated at 5 dpi, a time point when increased expression of three Bet v I/Major latex protein (MLP) homologous genes BvMLP1, BvMLP2 and BvML3 was observed in the partially resistant genotypes. Quantitative RT-PCR analysis on diseased sugar beet seedlings validated the activity of BvMLP1 and BvMLP3 observed in the transcriptome during challenge by R. solani. The three BvMLP genes were cloned and overexpressed in Arabidopsis thaliana to further dissect their individual contribution. Transgenic plants were also compared to T-DNA mutants of orthologous MLP genes. Plants overexpressing BvMLP1 and BvMLP3 showed significantly less infection whereas additive effects were seen on Atmlp1/Atmlp3 double mutants. The data suggest that BvMLP1 and BvMLP3 may contribute to the reduction of the Rhizoctonia root rot disease in sugar beet. Impact on the defense reaction from other differential expressed genes observed in the study is discussed. Keywords Arabidopsis · Defense genes · Major latex protein-like · Rhizoctonia solani · RNA-seq · Sugar beet
Introduction
Communicated by Stefan Hohmann. Louise Holmquist and Fredrik Dölfors contributed equally. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00438-020-01735-0) contains supplementary material, which is available to authorized users. * Christina Dixelius [email protected] 1
MariboHilleshög Research AB, Säbyholmsvägen 24, 26191 Landskrona, Sweden
2
Department of Plant Biology, Uppsala BioCenter, Linnean Center for Plant Biology, Swedish University of Agricultural Sciences, P.O. Box 7080, 75007 Uppsala, Sweden
3
Syngenta, Crop Protection, 9 Davis Drive, Research Triangle Park, NC 27709, USA
Beta vulgaris ssp. vulgaris, commonly known as sugar beet, is a dicot crop grown in the temperate zone with Europe and the USA as the major production regions (Draycott 2006). The crop is cultivated for its carbohydrate-enriched taproot. In addition to sugar, sugar beet is also a source for an array of carbohydrate-based products including biofuel (Duraisam et al. 2017)
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