Enhanced Human Bone Marrow Mesenchymal Stem Cell Chondrogenic Differentiation on Cold Atmospheric Plasma Modified Cartil
- PDF / 15,277,143 Bytes
- 6 Pages / 612 x 792 pts (letter) Page_size
- 3 Downloads / 197 Views
Enhanced Human Bone Marrow Mesenchymal Stem Cell Chondrogenic Differentiation on Cold Atmospheric Plasma Modified Cartilage Scaffold Wei Zhu1, Michael Keidar1, Lijie Grace Zhang1, 2 1 Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, DC, United States 2 Department of Medicine, The George Washington University, Washington, DC, United States ABSTRACT Articular cartilage is prone to degeneration and possesses extremely poor self-healing capacity due to its low cell density and absence of blood vessels. It has extensively reported tissue engineered scaffold can be a promising approach for cartilage repair. However, there still remains an inherent lack of desirable scaffolds that stimulate cartilage regrowth with appropriate functional properties. Therefore, in this study, we develop a biomimetic cartilage substitute comprising of electrospun polycaprolactone (PCL) with cold atmospheric plasma (CAP) modified cell favorable surface and sustained bioactive factor (bovine serum albumin (BSA) or transforming growth factor beta 1 (TGF-β1)) incorporated microspheres inside for improving stem cell chondrogenesis and cartilage regeneration. Scanning electron microscopy (SEM) analysis showed the drug delivery spheres homogeneously distribution in the fibrous scaffold. Furthermore, CAP treatment renders the scaffold’s surface more hydrophilic and results in more specific vitronectin adsorption as illustrated by contact angle and ELISA testing. Our results showed that the CAP treated scaffold can greatly improve growth and chondrogenic differentiation (such as increased glycosaminoglycan (GAG) synthesis) of human bone marrowderived mesenchymal stem cells (MSCs). INTRODUCTION Articular cartilage is unique tissue in human body which connects diarthrodial joints, lubricates surface between bones, transfers and withstands repetitive load over the lifetime. It is a thin layer tissue in the range of 1-4 mm depending on the location in human body, which makes it prone to degeneration aroused from trauma, disease, mechanical overloading [1, 2]. The limited self-repair capacity of natural cartilage leads scientists to draw aspiration from artificial intervention. 3D tissue engineered scaffolds present promise in regenerating injured cartilage. Among various tissue engineered scaffold fabrication techniques, electrospinning has gained increasing attention in cartilage repair [3]. It can create fibrous scaffolds with the diameter of micro to nano dimension to resemble natural extracellular matrix and porous architecture for enhancing cellular infiltration. On the other side, CAP is an ionized gas in which the ion temperature is close to room temperature, as opposed to typical high-temperature laboratory plasmas [4]. It can be induced in the presence of Helium with a high applied voltage. CAP is composed of various electrons, positive/negative ions, radicals, excited molecules, energetic photons (UV) and transient electric fields [5]. Give to the versatility of the CAP technique, CAP modification
Data Loading...
