Ethylene response of salt stressed rice seedlings following Ethephon and 1-methylcyclopropene seed priming
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ORIGINAL PAPER
Ethylene response of salt stressed rice seedlings following Ethephon and 1‑methylcyclopropene seed priming Sajid Hussain1 · Chunquan Zhu1 · Jie Huang1 · Jing Huang1 · Lianfeng Zhu1 · Xiaochuang Cao1 · Satyabrata Nanda1 · Maqsood Ahmed Khaskheli1 · Qingduo Liang1 · Yali Kong1 · Qianyu Jin1 · Junhua Zhang1 Received: 9 January 2020 / Accepted: 23 May 2020 © Springer Nature B.V. 2020
Abstract Salinity stress is one of the most devastating abiotic stresses affecting rice growth and productivity. Several phytohormones in rice contribute towards salinity response, including ethylene (ET). Furthermore, priming of rice seedlings with different chemicals has revealed new insights in rice stress physiology. Therefore, the hydroponic culture experiment under completely randomized design (CRD) factorial was conducted to study the effects of seed priming with two contrasting chemicals 1-Methylcyclopropene (1-MCP) and Ethephon with respect to ET production under salinity stress levels 0 (control, CK) and 25 mM NaCl (salinity stress, SS) on cultivar Zhongzheyou 1 (indica) with five replications. Results revealed that the use of 1-MCP and Ethephon improved overall rice seedling germination and growth as compared to the unprimed one under salinity stress. Early germination rate was found to be higher in the Ethephon primed seedlings. However, the late germination rate, overall seedling vigor index (SVI), total plant dry biomass (higher as 37.8 and 42% in 1-MCP-primed seeds, and 28.1 and 21.3% higher in Ethephon-primed seeds than no primed seeds under both salinity levels), other agronomic traits, and SPAD value were found to be higher in the 1-MCP primed seedlings. Further, the production of reactive oxygen species was comparatively higher in the Ethephon primed seedlings than the 1-MCP-primed seedlings. In addition, evaluation of ET levels and expression profiling (qRT-PCR) of some ET signaling genes revealed that Ethephon priming resulted in elevated ET concentration and upregulated ET signaling genes, including OsACS1 and OsEIN2. Overall, our results provide a basic idea of the role of rice seed priming with Ethephon and 1-MCP under salinity condition and their effects on the ET interplay.
Sajid Hussain, Chunquan Zhu have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10725-020-00632-1) contains supplementary material, which is available to authorized users. * Qianyu Jin [email protected]
Xiaochuang Cao [email protected]
* Junhua Zhang [email protected]
Satyabrata Nanda [email protected]
Sajid Hussain [email protected]
Maqsood Ahmed Khaskheli [email protected]
Chunquan Zhu [email protected]
Qingduo Liang [email protected]
Jie Huang [email protected]
Yali Kong [email protected]
Jing Huang [email protected]
1
Lianfeng Zhu [email protected]
State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang, China
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