Exosomes as Part of the Human Adipose-Derived Stem Cells Secretome- Opening New Perspectives for Cell-Free Regenerative
Human adipose-derived stem cells (hASCs) represent a great resource for regenerative medicine based on their accessibility, self-renewal potential, low immunogenicity, high proliferative rate and potential to differentiate on multiple lineages. Their secr
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Exosomes as Part of the Human Adipose-Derived Stem Cells SecretomeOpening New Perspectives for Cell-Free Regenerative Applications Sorina Dinescu, Alexandra Dobranici, Ramona Tecucianu, Aida Selaru, Roxana Balahura, Simona Ignat, and Marieta Costache Abstract
Human adipose-derived stem cells (hASCs) represent a great resource for regenerative medicine based on their accessibility, selfrenewal potential, low immunogenicity, high proliferative rate and potential to differentiate on multiple lineages. Their secretome is rich in chemokines, cytokines and protein growth factors that are actively involved in regenera-
Alexandra Dobranici and Ramona Tecucianu contributed equally with all other contributors. S. Dinescu (*) and M. Costache Department of Biochemistry and Molecular Biology, University of Bucharest, Bucharest, Romania The Research Institute of the University of Bucharest, Bucharest, Romania e-mail: [email protected] A. Dobranici, R. Tecucianu, and S. Ignat Department of Biochemistry and Molecular Biology, University of Bucharest, Bucharest, Romania A. Selaru and R. Balahura Department of Biochemistry and Molecular Biology, University of Bucharest, Bucharest, Romania Victor Babes National Institute of Pathology, Bucharest, Romania
tion processes. In addition, part of this secretome are also the exosomes (hASCexos), which display high content in proteins, messenger RNAs (mRNAs) and non-coding RNAs (ncRNAs). Due to their content, exosomes promote tissue regeneration by different mechanisms, either by activating or inhibiting several signaling pathways involved in wound healing, extracellular matrix remodeling, immunomodulation, angiogenesis, anti-apoptotic activity and cell migration, proliferation and differentiation. The use of hASC-exos may provide an improved alternative to standard therapies used in regenerative medicine, as a cell-free new approach with multiple possibilities to be modulated according to the patient needs. This review offers an updated overview on the functions and applications of hASC-exos in all areas of tissue regeneration, aiming to highlight to the reader the benefits of using hASCs in modern tissue engineering and regenerative medicine applications. Keywords
Exosomes · Human adipose-derived stem cells · miRNAs · Signaling pathways in regeneration · Tissue regeneration
S. Dinescu et al.
Abbreviations AFM AK2 AKI AKT Alix Arg-1 AT Bad Bax Bcl-2 BDNF bFGF Bim BMASCs BMEC BMP CD CM Col2A1 COX CSCs CSF-1R DDL4 Dvl ECM EGF EPCs ERK ESCRT EVs MVBs FBS FGF-1 FIZZ G-CSF GDNF hASCs HDFs HIPK2
atomic force microscopy adenylate kinase 2 acute kidney injury Protein kinase B ALG2-interacting protein X arginase-1 adipose tissue Bcl associated agonist of cell death Bcl-2-associated X protein B-cell lymphoma 2 brain-derived neurotrophic factor basic fibroblast growth factor Bcl-2-like protein 11 bone-marrow derived stem cells brain microvascular endothelial cells bone morphogenetic proteins cluster of differentiation conditioned medium type II collagen alpha 1 cyclooxygenase cancer st
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