Osteogenically-induced exosomes stimulate osteogenesis of human adipose-derived stem cells
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Osteogenically-induced exosomes stimulate osteogenesis of human adipose-derived stem cells Mengru Zhu . Yang Liu . Hongzhi Qin . Shuang Tong . Qiang Sun . Ting Wang . Hua Zhang . Mengying Cui . Shu Guo
Received: 5 April 2020 / Accepted: 21 September 2020 Ó The Author(s) 2020
Abstract Exosomes exhibit great therapeutic potential in bone tissue engineering. The study aimed to investigate whether the exosomes derived from human adipose-derived stem cells (hADSCs-Exos) during different time-span of osteogenic differentiation could promote osteogenesis. The appropriate concentrations of hADSCs-Exos to enhance the proliferation, migration and osteogenesis of hADSCs-Exos were also examined. PKH67 labelled hADSCs-Exos was used to detect the internalization ability of hADSCs. The osteogenic differentiation abilities of hADSCs after treatment with hADSCs-Exos was evaluated by Alizarin red staining (ARS). The proliferation and migration of hADSCs was examined by cell counting
Mengru Zhu and Yang Liu have contributed equally to this work. M. Zhu H. Qin Department of plastic surgery, The First Affiliated Hospital of Dalian Medical University, 222 Zhongshan Road, Dalian 116011, China Y. Liu School of Chemical Engineering, Dalian University of Technology, No. 2 Linggong Road, Dalian 116024, China S. Tong Q. Sun T. Wang H. Zhang M. Cui S. Guo (&) Department of Plastic surgery, The First affiliated Hospital of China Medical University, No 155 Nanjing North Street, Shenyang 110002, China e-mail: [email protected]
kit-8 and wound healing assay, respectively. The expression of exosomal surface markers and osteoblast-related protein of hADSCs was assessed by Western blot. PKH67-labelled exosomes were internalized by hADSCs after 4 h incubation. ARS showed that the amount of mineralized nodules in Exo1-14d group was significantly higher than that in Exo15-28d group. hADSCs-Exos could promote the proliferation and migration capacity of hADSCs. Western blot analysis showed that after hADSCs-Exos treatment, ALP and RUNX2 were significantly enhanced. Specially, the Exo1-14d group of 15 lg/mL significantly upregulated the expression of RUNX2 than the other exosomes treated groups. Our findings suggest that exosomes secreted by hADSCs during osteogenic induction for 1–14 days could be efficiently internalized by hADSCs and could induce osteogenic differentiation of hADSCs. Moreover, administration of Exo1-14d at 15 lg/mL promoted the proliferation and migration of hADSCs. In conclusion, our research confirmed that comprised of hADSCs-Exos and hADSCs may provide a new therapeutic paradigm for bone tissue engineering. Keywords Human adipose-derived stem cells Exosomes Osteogenic differentiation Bone tissue engineering Abbreviations hADSCs Human adipose-derived stem cells
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Cell Tissue Bank
hADSCsExos BMSCs MSCs TEM PM OM ARS DLS ALP ECM EVs CCK-8 FBS PBS DAPI RUNX2
Human adipose-derived stem cellsderived exosomes Bone marrow-derived mesenchymal stem cells Mesenchymal stem
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