Experimental Models of Cardiovascular Diseases Methods and Protocols
This book provides methodological information on establishing reliable and reproducible experimental models of cardiovascular diseases. Chapters detail practical protocols from expert laboratories focusing on cardiovascular research, leading to novel dis
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Kiyotake Ishikawa Editor
Experimental Models of Cardiovascular Diseases Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Experimental Models of Cardiovascular Diseases Methods and Protocols
Edited by
Kiyotake Ishikawa Icahn School of Medicine at Mount Sinai, New York, NY, USA
Editor Kiyotake Ishikawa Icahn School of Medicine at Mount Sinai New York, NY, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-8596-8 ISBN 978-1-4939-8597-5 (eBook) https://doi.org/10.1007/978-1-4939-8597-5 Library of Congress Control Number: 2018947193 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Cardiovascular disease continues to be the leading global cause of death. The number of patients suffering from these diseases is expected to further grow in the next decade due to the aging population worldwide as well as lifestyle changes in developing countries. Thorough research is ongoing to combat these diseases; however much remains to be elucidated before we fully understand the fundamental pathophysiological processes. Meanwhile, new therapeutic approaches are being actively explored in various areas including pharmacological, cell, gene, and device therapies. Common to both of these research areas is the importance of designing appropriate and reproducible experiments. Among the various factors, the disease model is the essential e
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