Expression analyses of miRNA Up-MIR-843 and its target genes in Ulva prolifera
- PDF / 713,308 Bytes
- 8 Pages / 595 x 842 pts (A4) Page_size
- 101 Downloads / 158 Views
Expression analyses of miRNA Up-MIR-843 and its target genes in Ulva prolifera Juanjuan Yang1, Dachun Yu1, Songdong Shen1* 1 Department of Cell Biology, School of Biology and Basic Medical, Soochow University, Suzhou 215123, China
Received 11 September 2019; accepted 22 November 2019 © Chinese Society for Oceanography and Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract
microRNAs (miRNA) families play a critical role in plant growth, development, and responses to abiotic stress. In this study, we characterized Up-miR-843 and its targets genes in Ulva prolifera responses to nitrogen depravation and heat stress. The data demonstrated that 184 target genes of Up-miR-843 could be successfully validated. N deficiency not heat stress stimulus induced increase in abundance of the Up-miR-843 while exhibited reverse expression of target genes, including cyclin A3 and cyclin L, which were strictly required for cell cycle progression. In addition, U. prolifera with highly expression of Up-miR-843 showed improved biomass, and photosynthesis compared with that under normal growth conditions. Thus, the N deprivation and heat responsive miRNAs might be a possible member mediating the expression of these target genes, which further regulated the growth of U. prolifera. Key words: cyclins, nitrogen deprivation, microRNA, U. prolifera, heat stress Citation: Yang Juanjuan, Yu Dachun, Shen Songdong. 2020. Expression analyses of miRNA Up-MIR-843 and its target genes in Ulva prolifera. Acta Oceanologica Sinica, 39(10): 27–34, doi: 10.1007/s13131-020-1657-2
1 Introduction Ulva prolifera is one of the most common bloom-forming macroalgae, which broken out since 2008 in the Yellow Sea (Lin et al., 2011) and have caused both ecological and economic impacts to coastal environments and human activities. These blooms of opportunistic macroalgae are generally explained by eutrophication caused by the increased nutrient (Conley et al., 2009; Pérez-Mayorga et al., 2011), optimal light, temperature and so on (McGlathery, 2001; Taylor et al., 2001). While physical forcing by winds and tides in shallow estuaries can lead to the rapid replacement of more saline, nutrientpoor marine water, higher surrounding temperature (Stumpf et al., 1993), Ulva still exhibited rapid nutrient uptake and subsequent growth. For example, U. lettuce maintained the ability to take up ammonium and nitrate under conditions of rapidly changing salinity (Lartigue et al., 2003), and nitrogen would be the limiting for the growth of U. curvata only at saturated light conditions (Coutinho and Zingmark, 1993). Therefore, we hold the view that Ulva might evolve self-regulating mechanisms to adapt to this abiotic stress. miRNAs are a group of the non-coding small RNA, which play vital roles in mediating plant growth, development, abiotic stress adaptation and so on (Jones-Rhoades et al., 2006; Voinnet, 2009). During past decade, various of the miRNAs that are involved in transducing nutrient signaling, including starvation of nitrogen (Gao et al., 2016; Paul e
Data Loading...
