Expression of H19 long non-coding RNA is down-regulated in oral squamous cell carcinoma
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Ó Indian Academy of Sciences ( 01234567 89().,-volV)( 01234567 89().,-volV)
Expression of H19 long non-coding RNA is down-regulated in oral squamous cell carcinoma SUPRIYA VISHWAKARMA1, RITU PANDEY1, RENU SINGH2, RAGINI GOTHALWAL3 and ASHOK KUMAR1* 1
Department of Biochemistry, All India Institute of Medical Sciences (AIIMS), Saket Nagar, Bhopal 462 020, India
2
Jawaharlal Nehru Cancer Hospital and Research Centre (JNCHRC), Idgah Hills, Bhopal, India 3
Department of Biotechnology, Barkatullah University, Hoshangabad Road, Bhopal, India *Corresponding author (Email, [email protected]) MS received 13 June 2020; accepted 23 October 2020
Long non-coding RNAs (lncRNAs) are a group of non-protein-coding RNAs which are longer than 200 nucleotides. LncRNAs play important roles in epigenetic modification, transcription and post-transcriptional regulation, maintenance of normal tissue development and differentiation. LncRNA could serve as a biomarker for diagnosis and prognosis as well as a molecular target for therapy in oral squamous cell carcinoma (OSCC). Therefore, we have determined the expression profile of 5-lncRNAs namely UCA1, TUG1, HOTAIR, MALAT1, and H19 by quantitative real-time PCR in tumor tissues and adjacent normal tissue of 32 OSCC patients. To determine the expression, methylation status and genomic alterations in lncRNAs across pancancer, TCGA datasets were analyzed by UALCAN, MEXPRESS and cBioPortal database. Then, we determined the association between lncRNA expression and clinicopathological attributes of patients by Spearman’s rank test. Expression of UCA1 and TUG1 genes was up-regulated in 54.83% and 53.12% OSCC tumors, respectively. Importantly, expression of MALAT1 and H19 was down-regulated in tumor tissues of 62.5% and 81.25% respectively of OSCC patients. Except for MALAT1, our experimental data showed concordance with the TCGA analysis. Expression of HOTAIR in OSCC tumors was positively correlated with tumor volume, whereas MALAT1 and H19 negatively correlated with the smoking status of patients. Keywords.
H19; long non-coding RNAs; oral cancer; oral squamous cell carcinoma; TCGA analysis
1. Introduction Head and neck cancer (HNC) is the sixth most common cancer in the world. HNC arises in the oral cavity, pharynx, larynx, paranasal cavity, nasal cavity and salivary glands (Bray et al. 2018). As per GLOBOCON 2018 data, the age-standardized rate per 100,000 for cancer of the lip and oral cavity in males is twice in developing countries compared to developed countries (Bray et al. 2018). The majority of oral cavity, oropharyngeal, hypopharyngeal and laryngeal cancers are squamous cell carcinoma (SCC) in histology and are referred to as oral squamous cell carcinoma
(OSCC). OSCC is the most common cancer in Indian men, accounting for one-sixth of all cancers (Mallath et al. 2014). The global incidence of OSCC is more than 350,000 (Bray et al. 2018), out of which approximately one-fourth of the cases are contributed by South-central Asia (Mallath et al. 2014). Rapid advance
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