Expression of internal reproductive barriers in a germplasm bank accession of the wild potato Solanum chacoense Bitter i
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RESEARCH ARTICLE
Expression of internal reproductive barriers in a germplasm bank accession of the wild potato Solanum chacoense Bitter in three ex situ regeneration cycles Anabela Poulsen Hornum . Elsa Lucila Camadro
Received: 6 January 2020 / Accepted: 23 September 2020 Ó Springer Nature B.V. 2020
Abstract Wild potatoes (Solanum sp.) are important reservoirs of desirable genes for potato breeding. Most of them are diploid and obligate outcrossers, with both sexual and asexual reproduction. Samples of natural populations are ex situ conserved as accessions in germplasm banks for basic and applied ends, and are regenerated when seed number, seed viability or both decrease. The possible action of within accession internal reproductive barriers is not considered in the current seed regeneration protocols, in which 20 to 25
This paper is part of a thesis by Anabela Poulsen Hornum in partial fulfillment of the requirements for the Doctor’s degree, Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Argentina.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10722-020-01034-y) contains supplementary material, which is available to authorized users. A. Poulsen Hornum E. L. Camadro (&) Unidad Integrada: Facultad de Ciencias Agrarias (FCA), Universidad Nacional de Mar Del Plata (UNMdP)Estacio´n Experimental Agropecuaria (EEA) ‘‘Domingo R. Pasquale’’, Instituto Nacional de Tecnologı´a Agropecuaria (INTA), Ruta Nacional 226 km 73.5, 7620 Balcarce, Buenos Aires, Argentina e-mail: [email protected]
plants are cultivated for the crossing work. Notwithstanding, the effective number of progenitors (Ne) in every seed regeneration cycle might be reduced in relation to the real number (N) of plants if internal reproductive barriers were acting; this process can eventually lead to gene erosion. To ascertain if Ne = N in seed regeneration of one randomly provided accession of S. chacoense Bitter, pollen viability and size, plus pollen-pistil relationships and number and type (according to endosperm development) of seeds/fruit were studied, respectively, at the individual plant and genotypic combination levels. Two types of barriers, pre-zygotic (pollen-pistil self- and crossincompatibility) and post-zygotic (male sterility and inviability and weakness of F1 hybrids) were detected in three growing seasons, indicating that not all genotypes in this accession made an equal allele contribution to the next generation. These results should be an alert for germplasm bank curators of wild potatoes and other crop wild relatives. Since the objective of germplasm banks is the ex situ maintenance of the natural genetic diversity, strategies need to be devised based on genetic premises. Keywords Effective number of progenitors Germplasm bank collections Parental genetic contribution Reproductive barriers Wild potatoes
A. Poulsen Hornum E. L. Camadro Consejo Nacional de Investigaciones Cientı´ficas y Te´cnicas (CO
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