Expression of RUNX2 and Osterix in Rat Mesenchymal Stem Cells during Culturing in Osteogenic-Conditioned Medium
- PDF / 1,569,873 Bytes
- 5 Pages / 594 x 792 pts Page_size
- 105 Downloads / 177 Views
571
Cell Technologies in Biology and Medicine, No. 2, August, 2020
Expression of RUNX2 and Osterix in Rat Mesenchymal Stem Cells during Culturing in Osteogenic-Conditioned Medium L. A. Pokrovskaya1, S. V. Nadezhdin2, E. V. Zubareva2, Yu. E. Burda3, and E. S. Gnezdyukova2
Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 2, pp. 112-117, June, 2020 Original article submitted December 11, 2019 We studied the expression of transcription factors RUNX2 and Osterix after addition of a concentrate of osteogenic-conditioned medium to the culture medium for osteogenic differentiation of mesenchymal stem cells (MSC). The obtained concentrate of osteogenic-conditioned medium containing a complex of bioactive substances with a molecular weight >10 kDa provided MSC differentiation into osteoblasts, which was confirmed by high level of expression of transcription factors RUNX2 and Osterix in comparison with the negative control. The highest expression of transcription factor Osterix was revealed on day 14 of MSC culturing in the presence of osteogenic supplement StemPro (positive control) and the studied concentrate of osteogenic-conditioned medium. Key Words: mesenchymal stem cells; osteoinduction; differentiation; osteogenic-conditioned medium Mesenchymal stem cells (MSC) hold great promise for cell therapy, tissue engineering, and regenerative medicine. One of the modern tasks of regenerative medicine is creation of bone grafts based on populations of living cells and paracrine factors [15]. Tissue engineering in situ, a new strategy for repairing bone defects [6], requires both biomimetic cell carriers and osteoinductors for in situ technologies. Isolation and use of paracrine factors are general approaches in enhancing the regeneration process based on MSC therapy [11]. The most widely used paracrine factors exhibiting osteoinductive properties are bone morphogenetic protein-1 (BMP), fibroblast growth factor (bFGF), insulin-like growth factor-1 (IGF-1), stromal cell factor 1 (SDF-1), etc. [14,16]. In parallel, the possibility of using osteogenicconditioned media for induction of osteoblastic differentiation of MSC is studied. It was shown that National Research Tomsk State University, Tomsk; 2Research Laboratory “Cellular, Assisted Reproductive, and DNA Technologies”, Belgorod State National Research University, Belgorod; 3Innovation Centre “Biruch — New Technologies”, Alekseevka, Belgorod region, Russia. Address for correspondence: [email protected]. S. V. Nadezhdin 1
osteogenic-conditioned medium from human periosteum-derived cells (hPDC) stimulated the expression of receptor activator of NF-κB ligand (RANKL) and promoted osteoclastogenesis [9]. Interesting results were demonstrated by a group of researchers demonstrating a decrease in the level of some cytokines, chemokines, and growth factors not exhibiting direct osteoinductive activity in the conditioned medium obtained after culturing of adipose tissue-derived stem cells (ASC) and stem cells from human exfoliated deciduous teeth (SHED) with ost
Data Loading...
