Extragonadal aromatization increases with time after ovariectomy in rats
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BioMed Central
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Extragonadal aromatization increases with time after ovariectomy in rats Hong Zhao†, Zhanzhuang Tian†, Junwei Hao and Boying Chen* Address: Department of Neurobiology and Integrative Medicine, Institute of Acupuncture Research (WHO Collaborating Centre for Traditional Medicine, Research Department of Acupuncture), Shanghai Medical College of Fudan University (Formerly Shanghai Medical University), P.O. Box 291, 138 Yi-Xue-Yuan Road, 200032 Shanghai, P. R. China Email: Hong Zhao - [email protected]; Zhanzhuang Tian - [email protected]; Junwei Hao - [email protected]; Boying Chen* - [email protected] * Corresponding author †Equal contributors
Published: 21 January 2005 Reproductive Biology and Endocrinology 2005, 3:6
doi:10.1186/1477-7827-3-6
Received: 27 November 2004 Accepted: 21 January 2005
This article is available from: http://www.rbej.com/content/3/1/6 © 2005 Zhao et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: The circulating estrogen concentration elevated gradually along with time after ovariectomy in rats. To explore the source of the increased circulation estrogen, the extragonadal aromatization as well as the synthesis of androgen in the adrenal cortex of the ovariectomized rats was evaluated. Methods: Female rats were divided into twelve groups: 1 month after ovariectomy (OVX1M), OVX2M, OVX3M, OVX4M, OVX5M, OVX6M; intact 1 month (INT1M), INT2M, INT3M, INT4M, INT5M, INT6M. The blood concentration of testosterone (T) was measured by radioimmunoassay. The mRNA expressions of P450 aromatase in the liver and subcutaneous abdominal (SA) adipose as well as the adrenal cytochrome P450 17 alpha hydroxylase/lyase (P450c17) were semiquantified by RT-PCR. The P450 aromatase protein expressions in the liver and SA adipose were detected by Western blot. Results: The blood E2 concentrations increased gradually along with time after ovariectomy in the rats. The 58-kDa aromatase protein and mRNA expressions normalized to β-actin in the OVX6M rats' SA adipose tissues showed higher levels than those from corresponding tissues in the INT6M (p < 0.05). And the ratios of aromatase mRNA and protein to β-actin in the OVX6M rats' liver tissues increased significantly compared with those in the OVX1M rats (p < 0.05). The ratio of adrenal P450c17 to beta-actin in the OVX6M increased markedly, and was higher than OVX1M (p < 0.05), though the blood concentration of T decreased significantly in all the ovariectomized rats (p < 0.05). Conclusion: Both the subcutaneous abdominal adipose tissues and the liver tissues contributed to the extragonadal aromatisation to promote the circulating E2 levels in the rats along with time after ovariectomy; the adrenal compensation might also be activated
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