Fabrication of graphene/gelatin/chitosan/tricalcium phosphate 3D printed scaffolds for bone tissue regeneration applicat
- PDF / 1,835,158 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 72 Downloads / 250 Views
ORIGINAL ARTICLE
Fabrication of graphene/gelatin/chitosan/tricalcium phosphate 3D printed scaffolds for bone tissue regeneration applications Huigen Lu1 · Xuekang Pan1 · Minjie Hu1 · Jianqiao Zhang1 · Yefeng Yu1 · Xuqi Hu1 · Kai Jiang2 Received: 31 December 2019 / Accepted: 27 October 2020 © King Abdulaziz City for Science and Technology 2020
Abstract The scaffold structures formed by the incorporation of reduced graphene oxide (RGO) nanomaterials can be studied to improve the 3D matrix properties in the applications of bone regeneration. On the other hand, RGO has delayed the incorporation into the mixtures that were determined to generate scaffolds on using 3D printing because of its poor colloidal stability of particles and weak solubility in water. Moreover, to produce RGO, the graphene oxide (GO) was treated with hydrazine hydrate solution, which was well known as a reducing agent that is highly hazardous. To outcome these drawbacks, an unique eco-friendly method was established to gather scaffolds that were 3D printed by the incorporation of RGO. This was obtained using the Gallic acid induced in situ reduction of the GO already existing on the gelatin/chitosan/tricalcium phosphate scaffolds. Moreover, scaffolds also exhibited antibacterial activity without conceding osteoblasts’ cell proliferation and viability. Furthermore, the obtained scaffolds showed good osteoblasts proliferation and viability indicating their possibility for applications in bone fracture healing. Keywords GO · Gelatin · Chitosan · Bone fracture · Scaffolds
Introduction Recently, critical-size bone defects augmentation, using tissue-engineering design based on the biological materials that employs different polymeric matrices, is being widely studied. Biocompatibility, versatility and biodegradability associated with the utilization of polymeric scaffolds suggested its extensive application in bone tissue engineering (BTE) (Schaffler et al. 2014; Davies and Hosseini 2000). Recapitulation of the extracellular matrix (ECM) present in bones reduces the hostile activity of the cells towards implanted graft materials and enhances osseo-integration (Gong et al. 2015). Various biological materials comprising * Xuqi Hu [email protected] * Kai Jiang [email protected] Xuekang Pan [email protected] 1
The Second Affiliated Hospital of Jiaxing University, Huangcheng North Road, Jiaxing 314000, China
Hand Surgery, 971th Hospital of PLA, No. 22 Minjiang Road, Qingdao 266071, Shandong, China
2
synthetic and natural polymers are utilized either as single or in combined forms to investigate proliferation, differentiation and interaction of the cells (Dhivya et al. 2015a, b; Locs et al. 2015; Logith Kumar et al. 2016). Among the utilized polymers in bone tissue engineering, chitosan (CS) is a naturally obtained polymer with residual monomers of D-glucosamine and N-acetyl-D-glucosamine associated with (Schaffler et al. 2014; Davies and Hosseini 2000; Gong et al. 2015; Dhivya et al. 2015a) β-glucosidic linkage which is frequently invest
Data Loading...
