First detection of canine parvovirus 2b DNA in a crab-eating fox pup ( Cerdocyon thous , Linnaeus, 1766)
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VETERINARY MICROBIOLOGY - SHORT COMMUNICATION
First detection of canine parvovirus 2b DNA in a crab-eating fox pup (Cerdocyon thous, Linnaeus, 1766) Caroline Giuseppa Spera 1 & Fernanda Louise Pereira Lavorente 1 & Elis Lorenzetti 1,2,3 & Gustavo de Calasans Marques 4 & Luana de Almeida Freitas 1 & Maíra Bonamin Martins 5 & Carlos Roberto Teixeira 4 & Amauri Alcindo Alfieri 1,2 & Alice Fernandes Alfieri 1,2 Received: 11 June 2020 / Accepted: 16 October 2020 # Sociedade Brasileira de Microbiologia 2020
Abstract The crab-eating fox (Cerdocyon thous) is a small wild mammal present in all Brazilian biomes and in some countries of South America. This study aimed to verify the involvement of viral infectious agents in the death of a wild crab-eating fox pup (Cerdocyon thous) in Brazil. The Center for Medicine and Research of Wild Animals of the Universidade Estadual Paulista received a free-living crab-eating fox aged approximately 21 days and apparently healthy. After 13 days, the animal presented anorexia, diarrhea, fever, prostration, and neurological signs progressing to death with an inconclusive diagnosis. In a retrospective study, tissue fragments stored at − 80 °C were used to identify nucleic acids from major canine viruses, such as canine parvovirus-2 (CPV-2), canine adenovirus A types 1 and 2, canid alphaherpesvirus 1, and canine distemper virus. The amplified product with the expected length for CPV-2 was obtained from the heart fragment. After performing nucleotide (nt) sequencing of the amplicon, it was possible to demonstrate that the crab-eating fox strain exhibited high (99.8%) nt identity with the CPV-2b prototype (CPV-39 strain). Additionally, deduced amino acid (aa) sequence analysis showed the GAT codon for the aa Asp (D) at position 426 of the CPV-2 viral protein VP2, which characterizes the subtype 2b. To the best of the authors’ knowledge, this report describes the first detection of CPV-2b DNA in tissue fragments from a crab-eating fox. Keywords Cerdocyon thous . Wild . Heart . CPV-2b
Responsible Editor: Fernando R. Spilki * Amauri Alcindo Alfieri [email protected] 1
Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Celso Garcia Cid Road, PR455 Km 380, P.O. Box 10011, Londrina, Paraná 86057-970, Brazil
2
Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Celso Garcia Cid Road, PR455 Km 380, P.O. Box 10011, Londrina, Paraná 86057-970, Brazil
3
Post Graduate Program in Animal Health and Production, Universidade Pitágoras Unopar, Arapongas, Paraná, Brazil
4
Center for Medicine and Research of Wild Animals, Veterinary Hospital, Universidade Estadual Paulista - Julio de Mesquita Filho, P.O. Box 560, Botucatu, São Paulo 18618-000, Brazil
5
Animal Pathology, Veterinary Hospital, Universidade Estadual Paulista - Julio de Mesquita Filho, P.O. Box 560, Botucatu, São Paulo 18618-000, Brazil
Introduction The crab-eating fox (Cerdocyon thous, Linnae
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