First Expressed TFome of Physic Nut ( Jatropha curcas L.) After Salt Stimulus
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ORIGINAL PAPER
First Expressed TFome of Physic Nut (Jatropha curcas L.) After Salt Stimulus George André de Lima Cabral 1 & Eliseu Binneck 2 & Marislane Carvalho Paz de Souza 1 & Manassés Daniel da Silva 1 & José Ribamar Costa Ferreira Neto 1 & Marcelo Francisco Pompelli 3 & Laurício Endres 4 & Éderson Akio Kido 1
# Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Physic nut (Jatropha curcas L.), a small oleaginous tree spontaneously occurring in arid and semi-arid tropical regions, is a sustainable and renewable energy source for biodiesel. However, the J. curcas yield in such areas should consider soil salinity and its consequences. Transcription factor (TF) proteins recognize cis-regulatory elements in promoters of genes to be expressed. In the present work, differentially expressed genes (DEGs) encoding putative TFs from physic nut plants responding to NaCl (150 mM), after 3 h of exposition, covered 23 TF families. The expressed profiles of members from AP2/ERF and NAC families basically showed induction after the salt stimulus, while members of bHLH, FHY3-FAR1, and ARF families presented repression. Concerning the induced TF DEGs, the gene ontology (GO) enrichment analysis highlighted terms related to abiotic stress responses, while those terms representing the repressed TF DEGs stood out the basal metabolism. In turn, the TF enrichment analysis predicted those TFs targeting promoters of induced TF DEGs. Some of the enriched TFs may be good candidates as transgenes in transgenic events. Also, RT-qPCR analyses validated the up-regulation of six TF DEGs (RAV1, ERF9, ZAT12, PTI5, MYB340, and BZIP4) of eight candidates selected from the expressed TFome. The generated data could help breeders to better understand the molecular basis of physic nut plants responding to salinity, to select potential candidates for transgenic studies, as well as to develop functional molecular markers to assist selection steps in breeding programs. Keywords Jatropha curcas . RNA-Seq . Transcriptome . Abiotic stress . Salinity Key Messages • First TFome expressed by J. curcas roots after 3 h of NaCl exposition (150 mM) • TFome covering 148 differentially expressed genes (DEGs) of 23 TF families • TF BPC6 was predicted to regulate more than 40 induced DEGs of J. curcas plants responding to the salt stimulus • Eight TF DEGs evaluated by a second gene expression technique (RTqPCR analysis) Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11105-019-01187-w) contains supplementary material, which is available to authorized users. * Éderson Akio Kido [email protected] George André de Lima Cabral [email protected] Eliseu Binneck [email protected] Marislane Carvalho Paz de Souza [email protected] Manassés Daniel da Silva [email protected]
José Ribamar Costa Ferreira Neto [email protected] Marcelo Francisco Pompelli [email protected] Laurício Endres [email protected] Extended author information available on the last page of
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