Profiling the Developing Jatropha curcas L. Seed Transcriptome by Pyrosequencing
- PDF / 328,304 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 68 Downloads / 237 Views
Profiling the Developing Jatropha curcas L. Seed Transcriptome by Pyrosequencing Andrew J King & Yi Li & Ian A Graham
Published online: 28 January 2011 # Springer Science+Business Media, LLC. 2011
Abstract Jatropha curcas L. has received much attention recently as a potential oilseed crop for the production of renewable oil. Despite the interest in this crop, relatively little is known on the molecular biology of this species compared with more established oilseed crops. To gain a more detailed understanding of the processes involved in deposition of oil and protein within Jatropha seeds, we conducted high-throughput sequencing analysis of the transcriptome of developing J. curcas seeds using 454 sequencing. A single sequencing run yielded 195,692 sequences (46 Mbp) of raw sequence data. Assembly of this sequence data produced 12,419 contigs and 17,333 singletons. BLASTX searches of the contigs revealed that storage proteins were the most abundant transcripts. Oleosins, ribosomal proteins, metallothioneins and late embryogenesis abundant proteins were also highly represented. Curcin, a type-I ribosome-inactivating protein, accounted for 0.7% of the transcriptome. No transcripts for type-II ribosome-inactivating proteins were found, suggesting that these are not present in the seeds of J. curcas. To test the power of 454 sequencing compared to conventional gene sequencing as a tool for gene discovery, a search of the homologues for genes involved in the Electronic supplementary material The online version of this article (doi:10.1007/s12155-011-9114-x) contains supplementary material, which is available to authorized users. A. J. King : Y. Li : I. A. Graham (*) Centre for Novel Agricultural Products, Department of Biology, University of York, Heslington York YO10 5DD, UK e-mail: [email protected] A. J. King e-mail: [email protected] Y. Li e-mail: [email protected]
conversion of sucrose to triacylglycerol was conducted. Hits for all the known genes in this process were obtained. Pyrosequencing of the J. curcas developing seed transcriptome has provided a valuable increase in the amount of sequence data currently available for this species. The sequence data will be of great use to those engaged in J. curcas research and crop improvement. Keywords Biodiesel . Expressed sequence tags . Jatropha curcas . Developing seeds
Introduction In recent years, much interest has been generated in the potential of Jatropha curcas L. as a perennial oilseed crop for cultivation in tropical and sub-tropical regions. Many plantations are now being established in Asia, Africa and Latin America for the production of biodiesel, and J. curcas is therefore likely to become an increasingly important oilseed crop over the next decade [1, 2]. J. curcas also has a history of cultivation under a number of less intensive agricultural systems. It can be used as a fencing crop, as a shade crop for plants such as vanilla or for controlling soil erosion [3]. Despite the interest in this species, relatively little is presently known about the molecular bi
Data Loading...
