First report of powdery mildew of Origanum onites caused by Golovinomyces biocellatus in Turkey
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First report of powdery mildew of Origanum onites caused by Golovinomyces biocellatus in Turkey Güliz Tepedelen Ağaner 1 & Ceren Cer 1 & Barbaros Çetinel 1 Received: 17 February 2020 / Accepted: 9 October 2020 # Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2020
Keywords Origanum onites . Pot marjoram . Golovinomyces biocellatus
Origanum onites L. (pot marjoram), Lamiaceae, is used for its aromatic and medicinal properties. Powdery mildew symptoms were observed in Spring 2014, in the Denizli province. Disease incidence was approximately 73%. Both adaxial and abaxial leaf surfaces were covered with white mycelium and conidia. Mycelium was also seen growing on the stem surface. As the disease progressed, infected leaves turned yellow, and eventually fell off. Conidia were elliptical, hyaline and measured 34.5 × 18.1 μm, while conidiophores were erect and measured 78.7 × 9.0 μm. Chasmothecia were not detected and fibrosin bodies were absent. Based on anamorph, morphology, the fungus was identified as Golovinomyces biocellatus (Ehrenb) V.P.Heluta (Braun 1987). The internal transcribed spacer (ITS) region of rDNA was amplified using the primers ITS1/ITS4 and sequenced (White et al. 1990) (GenBank Accession No. KU642024). The 560-bp amplicon obtained by PCR reaction was sequenced and showed 100% similarity to G. biocellatus (JN594608.1). For pathogenicity tests, a conidial suspension (6 × 104 CFU/ml) was prepared from leaves of diseased pot marjoram plant and inoculated onto leaves of five plants while the same number of noninoculated plants served as a control. Inoculated and control plants were covered with plastic bags and incubated in 70–
* Güliz Tepedelen Ağaner [email protected] 1
Plant Protection Research Institute-Bornova, Izmir, Turkey
80% humidity chamber for two days at 24 °C. They were left to grow normally. First symptoms appeared in ten days after inoculation but they did not appear on control plants. Golovinomyces biocellatus on O. vulgare has been reported in Italy, Argentina and it has been reported on rosemary plants in Turkey (Farr and Rossman 2020). However, there is no record of powdery mildew on O. onites. To our knowledge, this is the first report of G. biocellatus on O. onites in Turkey.
References Braun U (1987) A monograph of the Erysiphales (powdery mildews). Beihefte zur Nova Hedwigia 89:1–700 Farr DF, Rossman AY (2020) Fungal databases. U.S. National Fungus Colections, ARS, USDA. Retrieved March 26, 2020, https://nt.arsgrin.gov/fungaldatabases/. Accessed 10 Jun 2020 White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for Phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols. Academic Press, San Diego, pp 315–322 Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
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