First report on molecular basis of potato leaf roll virus (PLRV) aggravation by combined effect of tuber and prevailing
- PDF / 1,228,037 Bytes
- 4 Pages / 595.276 x 790.866 pts Page_size
- 56 Downloads / 122 Views
BMC Research Notes Open Access
RESEARCH NOTE
First report on molecular basis of potato leaf roll virus (PLRV) aggravation by combined effect of tuber and prevailing aphid Ravi Ranjan Kumar1, Mohammad Ansar2, Kumari Rajani3, Jitesh Kumar1 and Tushar Ranjan1*
Abstract Objective: The Potato Leaf Roll Virus (PLRV) is one of the most devastating virus causing severe yield losses worldwide in potato. The comprehensive observations were made to study the PLRV infestation in major potato growing areas of Bihar (India) and further detailed molecular basis of PLRV aggravation was established. Results: Although aphids population were found comparatively lower with maximum symptomatic plants, our molecular data further confirms the presence of PLRV in all possible symptomatic tissues such as tubers, shoots and leaves. For the first time, we have proposed molecular basis of aggravation of PLRV, where tuber acts as a reservoir during off-season and further transmitted by aphids. Keywords: Potato leaf roll virus (PLRV), Tuber, Reservoir, Aphid, Virus transmission Introduction The potato crop is severely affected by various biotic stresses and among which viruses play a significant contribution in terms of huge loss in crop yield worldwide including India. Potato is affected by deadly viruses especially more potential infection via seed tubers due to the vegetative reproduction of the crop. More than 40 viruses and viroids hamper the cultivation of potato across the globe [1, 2]. The crop is infected by more than 30 RNA viruses, out of which 13 are mainly transmitted by aphids. Potato leaf roll virus (PLRV), belongs to genus Polerovirus and family Luteoviridae, is a widely spread potato virus worldwide and responsible for more than 20 million tonnes yield loss (up to 90%) globally [3]. PLRV is the only transmitted by aphids, namely, Myzus persicae. It is widely multiplied in the phloem tissue and the symptoms of disease reflect this position [4]. Because potato is *Correspondence: [email protected] 1 Department of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Sabour, Bhagalpur 813 210, India Full list of author information is available at the end of the article
a vegetatively propagated crop, once it gets infected with viruses, they can easily disseminate in the progeny tubers. These viruses are found in single or most of the time as a mixed infection within the potato crops. Tubers used for planting in next season can harbor latent viruses that subsequently reduce emergence, plant vigor and yield. All daughter tubers produced by infected mother tubers (secondary infection) will also get infected via systemic translocation of the virus during growth [5, 6]. Although, potato leaf roll disease is widespread throughout India but it is extremely critical in eastern plain zone, where population of vectors are very high in mid-February and March. In subsequent months, the higher temperature does not support the survival and growth of the aphids, makes them unavailable for potato host plants wh
