Fluorometric determination of aflatoxin B1 using a labeled aptamer and gold nanoparticles modified with a complementary
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ORIGINAL PAPER
Fluorometric determination of aflatoxin B1 using a labeled aptamer and gold nanoparticles modified with a complementary sequence acting as a quencher Chao Wang 1,2 & Yapiao Li 1,2 & Ce Zhou 1 & Qiang Zhao 1,2 Received: 17 May 2019 / Accepted: 16 September 2019 # Springer-Verlag GmbH Austria, part of Springer Nature 2019
Abstract A fluorometric aptamer based assay is described for rapid and sensitive detection of aflatoxin B1 (AFB1). It is making use of a fluorescein (FAM) labeled anti-AFB1 aptamer and complementary DNA-modified gold nanoparticles (GNPs). In the absence of AFB1, the FAM-labeled aptamers hybridize with complementary DNA strands that were covalently immobilized on GNPs. This results in quenching of the green fluorescence (with excitation/emission peaks at 485/525 nm). In the presence of AFB1, the aptamer probe binds AFB1 and is released from the GNPs. Hence, fluorescence is restored. Under optimized conditions, AFB1 in the concentration range from 61 pM to 4.0 μM can be detected, and the detection limit is 61 pM. This assay is highly selective for AFB1. It was applied to the determination of AFB1 spiked into 50-fold diluted wine and 20-fold diluted beer. Keywords Fluorescent probe . Mycotoxin . Food safety . Envrionmental analysis . Fluorophore . Nanomaterials . Fluorescence quenching . Nanoprobe
Introduction Aflatoxins, one category of the most toxic mycotoxins, have attracted wide attentions due to their harmful effects on food safety and human health [1, 2]. Aflatoxin B1 (AFB1) is the most common and toxic component of aflatoxins, which may cause severe diseases such as liver cirrhosis, necrosis, and carcinoma in human beings and animals [3, 4]. The International Agency for Research in Cancer (IARC) had classified AFB1 into Group 1 carcinogen [5]. Lots of agricultural products (e.g., groundnut, corn, wheat and peanut) and food may suffer from AFB1 contamination [6]. Therefore, it is essential to develop sensitive and reliable analytical methods for detecting AFB1. Chromatography, mass spectrometry, and Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-019-3838-2) contains supplementary material, which is available to authorized users. * Qiang Zhao [email protected] 1
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
2
University of Chinese Academy of Sciences, Beijing 100049, China
immunoassays are commonly used methods for AFB1 detection, and they may have some limitations for rapid detections of AFB1 [7–9]. The preparation of antibody usually needs high cost, and antibody stability may affect the application. Aptamers are oligonucleotides that can selectively bind with a broad range of targets from inorganic ions to organic molecules, peptides, proteins and even cells [10–12]. In comparison with antibodies, aptamers show advantages in easy preparation, low cost, good stability, and ease of labeling with fun
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