Control of the rubber anthracnose fungus Colletotrichum gloeosporioides using culture filtrate extract from Streptomyces
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ORIGINAL PAPER
Control of the rubber anthracnose fungus Colletotrichum gloeosporioides using culture filtrate extract from Streptomyces deccanensis QY-3 Liushuang Gu . Kai Zhang . Nan Zhang . Xiaoyu Li . Zhiqiang Liu
Received: 21 April 2020 / Accepted: 10 August 2020 Ó Springer Nature Switzerland AG 2020
Abstract Colletotrichum gloeosporioides is a main cause of rubber anthracnose, which results in a huge loss for the natural rubber industry. In this study, an actinomycete strain QY-3 was isolated and had good antagonistic activity against C. gloeosporioides with an inhibition rate of 86.6%. Strain QY-3 was identified as Streptomyces deccanensis preliminarily. Millet medium was selected as the optimal fermentation broth for antifungal metabolites production by S. deccanensis QY-3. The culture filtrate extract (CFE) from the millet broth of S. deccanensis QY-3 exhibits broad-spectrum antifungal activity against plant pathogenic fungi, and its EC50 inhibiting the mycelial growth of C. gloeosporioides is 6.3 lg/mL. The CFE has good thermal and pH stabilities, and it can break the hyphae and inhibit the conidial germination of C. gloeosporioides. 100 lg/mL of CFE had an obvious Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10482-020-01465-8) contains supplementary material, which is available to authorized users. L. Gu K. Zhang N. Zhang X. Li Z. Liu School of Life and Pharmaceutical Sciences, Hainan University, Haikou, China X. Li (&) Z. Liu (&) Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan University, Haikou, China e-mail: [email protected] Z. Liu e-mail: [email protected]
control effect on rubber anthracnose, and the control efficacy was 63.7% on 5 days after inoculation. Two compounds with inhibitory effects on C. gloeosporioides, anthranilic acid and sangivamycin, were isolated from the CFE. The MICs of both compounds against C. gloeosporioides were 29.3 and 23.0 lg/mL respectively. In conclusion, the CFE from S. deccanensis QY-3 has great potential to be a promising fungicide for rubber anthracnose. Keywords Rubber anthracnose Colletotrichum gloeosporioides Streptomyces deccanensis Culture filtrate extract Sangivamycin
Introduction Rubber anthracnose is one of the most important leaf diseases of rubber tree (Hevea brasiliensis), which is widely distributed in rubber planting areas around the world (Liu et al. 2018a). It can damage rubber seedlings, young trees and mature trees in cutting time (Jean et al. 2005). In serious cases, it may cause repeated defoliation and young shoots back to wither, delaying cutting time, and significantly reducing the rubber production (Cai et al. 2013). Colletotrichum gloeosporioides, the causal agent of rubber anthracnose, has a wide range of hosts such as mango, avocado, papaya and chili (Phoulivong et al. 2010; Liu
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Antonie van Leeuwenhoek
et al. 2018a). During establishment and colonization of host plants, C. gloeos
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