GbMYBR1 from Ginkgo biloba represses phenylpropanoid biosynthesis and trichome development in Arabidopsis
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ORIGINAL ARTICLE
GbMYBR1 from Ginkgo biloba represses phenylpropanoid biosynthesis and trichome development in Arabidopsis Xiaojia Su1,2,3 · Yaying Xia1,2,3 · Wenbo Jiang1 · Guoan Shen4 · Yongzhen Pang1 Received: 29 June 2020 / Accepted: 18 September 2020 © The Author(s) 2020
Abstract Main Conclusion GbMYBR1, a new type of R2R3-MYB repressor from Ginkgo biloba, displayed pleiotropic effects on plant growth, phenylpropanoid accumulation, by regulating multiple related genes at different levels. Abstract Ginkgo biloba is a typical gymnosperm that has been thriving on earth for millions of years. MYB transcription factors (TFs) play important roles in diverse processes in plants. However, the role of MYBs remains largely unknown in Ginkgo. Here, an MYB TF gene from Ginkgo, designated as GbMYBR1, was found to act as a repressor in multiple processes. GbMYBR1 was mainly expressed in the leaves of Ginkgo. Over-expression of GbMYBR1 in Arabidopsis thaliana led to growth retardation, decreases in lignin content, reduced trichome density, and remarkable reduction in anthocyanin and flavonol contents in leaves. Proanthocyanidin content was decreased in the seeds of transgenic Arabidopsis, which led to light-brown seed color. Both qPCR and transcriptome sequencing analyses demonstrated that the transcript levels of multiple genes related to phenylpropanoid biosynthesis, trichome formation, and pathogen resistance were down-regulated in the transgenic Arabidopsis. In particular, we found that GbMYBR1 directly interacts with the bHLH cofactor GL3 as revealed by yeast two-hybrid assays. Our work indicated that GbMYBR1 has pleiotropic effects on plant growth, phenylpropanoid accumulation, and trichome development, mediated by interaction with GL3 or direct suppression of key pathway genes. Thus, GbMYBR1 represents a novel type of R2R3 MYB repressor. Keywords GbMYBR1 · Ginkgo biloba · Phenylpropanoids · Repressor · Transcriptional regulation · Trichome
Communicated by Dorothea Bartels. Xiaojia Su and Yaying Xia have contributed equally to this paper. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00425-020-03476-1) contains supplementary material, which is available to authorized users.
Abbreviations ANS Anthocyanin synthase bHLH Basic helix-loop-helix DFR Dihydroflavonol reductase EGL3 Enhancer of Glabra 3 F3H Flavanone 3-hydroxylase FLS Flavonol synthase GL1 Glabrous 1 GL3 Glabrous 3
* Yongzhen Pang [email protected]
1
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Xiaojia Su [email protected]
2
Yaying Xia [email protected]
Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
3
Wenbo Jiang [email protected]
University of Chinese Academy of Sciences, Beijing 100049, China
4
The Institute of Medicinal Plant Development, Beijing 100193, China
Guoan Shen [email protected]
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