Gene Profiling of Compatible and Incompatible Interactions between Potato and Phytophthora infestans
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Gene Profiling of Compatible and Incompatible Interactions between Potato and Phytophthora infestans Jie Liu 1,2,3 & Zhuo Yu 1 & Yu Yang 4 & Yanhong Ma 1 & Xiaoxia Yu 1 & Wei Wei 3 & Changhai Song 2 & Xiao Guo 4 & Xiaohui Yang 4 Accepted: 5 July 2020 / Published online: 14 July 2020 # The Potato Association of America 2020
Abstract Potato late blight, caused by the oomycete pathogen, Phytophthora infestans de Bary, is one of the most devastating plant diseases in the world. It is of great importance to screen disease resistance germplasms and elucidate the molecular mechanisms underlying the resistance. In a previous study, Huasong 7, a tetraploid potato variety, was identified to possess resistance to multiple P. infestans races at various levels. In the present study, Huasong 7 was inoculated with an incompatible isolate 428–2 and a compatible P. infestans isolate T30–4, and gene expression was profiled at 0, 24, 48, and 72 h post inoculation. The results showed that more gene expression differentiation was observed in the compatible interaction induced by T30–4 than that in the incompatible interaction induced by 428–2. The differentially expressed genes induced by 428–2 were significantly enriched in GO terms associated with immune responses and the regulation of defense responses, as well as KEGG pathways related to antioxidant activities, for example, the glutathione metabolism. In contrast, differentially expressed genes induced by T30–4 were significantly enriched in GO terms associated with transcription, translation, and biosynthesis of macromolecules, as well as disease resistance related KEGG pathways, such as phosphoinositide metabolism, phospholipid signaling system, and receptormediated endocytosis. Our findings indicated that Huasong 7 responds to incompatible P. infestans isolate through disease resistance and host defense related pathways; while it responds to compatible isolate T30–4 with basic responses. It is also possible that T30–4 contains other unknown Avr genes. In summary, our study could provide an important reference for a better understanding of potato late blight resistance mechanisms of both compatible and incompatible interactions. Resumen El tizón tardío de la papa, causado por el patógeno oomiceto Phytophthora infestans de Bary, es una de las enfermedades de plantas más devastadoras en el mundo. Es de gran importancia probar resistencia a la enfermedad de germoplasmas y elucidar los Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12230-020-09789-x) contains supplementary material, which is available to authorized users. * Zhuo Yu [email protected]
Changhai Song [email protected]
* Xiaohui Yang [email protected]
Xiao Guo [email protected]
Jie Liu [email protected]
1
Agricultural College, Inner Mongolia Agricultural University, Hohhot 010000, China
Yu Yang [email protected]
2
Inner Mongolia HuaSong Agricultural Technology Co., Ltd, Chifeng 024000, China
3
HuaSong Seed Industry (Beijing) co. LTD, Beijing 10008
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