Cancer Gene Profiling Methods and Protocols
This second edition volume brings together the experiences of leading scientists in the discipline of cancer gene profiling. Because cancer genes can be profiled in many different ways, Cancer Gene Profiling: Methods and Protocols, Second Edition explores
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Robert Grützmann Christian Pilarsky Editors
Cancer Gene Profiling Methods and Protocols Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Cancer Gene Profiling Methods and Protocols Second Edition
Edited by
Robert Grützmann Department of Surgery, Universitätsklinikum Erlangen, Erlangen, Germany
Christian Pilarsky Department of Surgery, TU-Dresden, Dresden, Germany
Editors Robert Grützmann Department of Surgery Universitätsklinikum Erlangen Erlangen, Germany
Christian Pilarsky Department of Surgery TU-Dresden Dresden, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3203-0 ISBN 978-1-4939-3204-7 (eBook) DOI 10.1007/978-1-4939-3204-7 Library of Congress Control Number: 2015958099 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Science is the facilitator between imagination and reality —(Anonymous)
Since the last edition of this book, the pace of technology has accelerated. The major cancer genome projects have been finished and shown that next-generation sequencing is the winning technology for high-throughput expression profiling. In this book we have brought together the experiences of leading scientists in the discipline of cancer gene profiling. We have included different techniques, since cancer genes can be profiled in different ways. Such different approaches are needed to understand the key stages of cancer development, as using only one technique would be insufficient. Therefore this book attempts to give an overview of the state-of-the-art methods, which wi
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