Genetic Diversity Assessment of a Chinese Medicinal Endemic Species, Aspidopterys obcordata var. obcordata , by Combined
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Genetic Diversity Assessment of a Chinese Medicinal Endemic Species, Aspidopterys obcordata var. obcordata, by Combined Molecular Marker Methods (ISSR & SRAP) Mei‑Fang Song1 · Yan‑Hong Guan1 · Yue Zhang1 · Hai‑Tao Li1 · Li‑Xia Zhang1 · Zhong‑Lian Zhang1 Received: 25 October 2018 / Accepted: 18 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Aspidopterys obcordata var. obcordata, a medicinal plant endemic to China, is a narrowly distributed species and wild resources are extremely limited. To evaluate the genetic variability and degree of genetic divergence of A. obcordata var. obcordata, and to make rational scientific decisions on its harvest and germplasm conservation, we collected 122 samples from across nearly all of its distribution area and studied genetic diversity using inter-simple sequence repeats (ISSRs), sequencerelated amplified polymorphisms (SRAPs), and a method combining the two techniques. The results revealed the high genetic diversity of A. obcordata var. obcordata, mainly due to its intra-population diversity, and the top two populations with the highest levels of intra-population diversity were ML and DH, individuals of which can serve as excellent germplasm candidates during the processing of germplasm screening and conservation. In general, the combining method was prior to the ISSR analyses and SRAP analyses results, except for a slight difference in the genetic structure of individual populations. Therefore, we suggest that a combination analysis of the two marker methods is ideal for evaluating the genetic diversity and genetic relationships of A. obcordata var. obcordata. Keywords A. obcordata var. obcordata · ISSR · SRAP · Genetic diversity · Genetic relationships
Li-Xia Zhang and Zhong-Lian Zhang have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s1052 8-020-10001-2) contains supplementary material, which is available to authorized users. * Li‑Xia Zhang [email protected] * Zhong‑Lian Zhang [email protected] Extended author information available on the last page of the article
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Biochemical Genetics
Abbreviations ISSR Inter-simple sequence repeat SRAPs Sequence-related amplified polymorphisms UPGMA Unweighted pair group method with arithmetic means AMOVA Analyses of molecular variance PCA Principal component analyses
Introduction Aspidopterys obcordata var. obcordata (C. B. Clarke) Ridley, a medicinal plant of the family Malpighiaceae, has been extensively used as a traditional medicine to treat acute and chronic nephritis, pyelonephritis, cystitis, urinary tract infections, kidney calculi, and gallstones (Food and drug administration of Yunnan province 2005), and can also effectively reduce the recurrence rate of stones (Mei-fang et al. 2015a,b, 2016; Yu La-dui and Jian-bo 2009; Zhi-gang 2010). Studies have shown that tannins and triterpenoids may be the main anti-calculus constituents of A. obcordata (Xiao-hua et al. 2014).
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