Genomic sequencing and characterization of a novel group of canine bufaviruses from Henan province, China
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Genomic sequencing and characterization of a novel group of canine bufaviruses from Henan province, China Ran Shao1,2,3 · Feiyan Zheng1,2,3 · Siqi Cai1,2,3 · Jinzhao Ji1,2,3 · Zixin Ren1,2,3 · Jiawei Zhao1,2,3 · Liyan Wu1,2,3 · Jiajun Ou1,2,3 · Gang Lu1,2,3 · Shoujun Li1,2,3 Received: 21 May 2020 / Accepted: 22 July 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract Canine bufavirus (CBuV) is a novel protoparvovirus of dogs that was first reported in 2018 in Italy. The prevalence and genetic diversity of CBuV in China are not clear. In this study, a total of 115 canine fecal samples were collected from northern China in 2019, and two of the samples tested positive for CBuV DNA by PCR. These two field CBuV strains were designated Henan38 and Henan44. The complete genomic sequences of Henan38 and Henan44 were obtained by gap-filling PCR, sequenced, and assembled. Phylogenetic analysis demonstrated that the two strains clustered together in a novel group that was distant from previously reported CBuV strains. This study will strengthen our understanding of the epidemiology and genetic diversity of CBuV in China. Bufaviruses (BuVs) are members of the genus Protoparvovirus and the family Parvovirinae [1]. Their genome consists of a single strand of DNA of 4.5–5.5 kb with complex hairpin structures at the 5′ and 3′ ends and encodes nonstructural protein 1 (NS1) and viral structural proteins 1 and 2 (VP1 and VP2) in most cases [2]. The first BuV was discovered in 2012 in fecal samples from children suffering from diarrhea. Since then, sporadic human cases have been reported in several countries at frequencies ranging from 0.27% to Handling Editor: Ana Cristina Bratanich Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-020-04785-2) contains supplementary material, which is available to authorized users. * Gang Lu [email protected] * Shoujun Li [email protected] 1
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, Guangdong Province, People’s Republic of China
2
Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou 510642, Guangdong Province, People’s Republic of China
3
Guangdong Technological Engineering Research Center for Pet, Guangzhou 510642, Guangdong Province, People’s Republic of China
4.0%. Protoparvoviruses related to BuVs have been detected in other mammals, such as pigs, dogs, bats, rats, shrews, and nonhuman primates [3–8]. Canine bufavirus (CBuV) was first reported in 2018 in samples from dogs with enteric and respiratory symptoms in Italy. The prevalence rate in previous reports ranged from 2.5% to 31%. To date, there have been only two reports of CBuV in southern China [5, 9]. Hence, the epidemiological and molecular data on CBuV in China are limited. In this study, CBuV was identified for the first time in fecal samples collected from domestic dogs in northern China and further characterized by
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