H 2 S induces NO in the regulation of AsA-GSH cycle in wheat seedlings by water stress
- PDF / 254,186 Bytes
- 7 Pages / 595.276 x 790.866 pts Page_size
- 20 Downloads / 160 Views
SHORT COMMUNICATION
H2S induces NO in the regulation of AsA-GSH cycle in wheat seedlings by water stress Changjuan Shan 1 & Baoshi Wang 1 & Haili Sun 1 & Shang Gao 1 & Hua Li 2 Received: 8 December 2019 / Accepted: 9 April 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract In current study, we investigated the relationship between hydrogen sulfide (H2S) and nitric oxide (NO) in the regulation of ascorbate-glutathione (AsA-GSH) cycle in wheat seedlings by water stress. Findings showed that water stress significantly stimulated the production of H2S and NO, the transcript levels and activities of enzymes in AsA-GSH cycle, as well as malondialdehyde (MDA) production and electrolyte leakage, but significantly decreased AsA/DHA and GSH/GSSG. Meanwhile, water stress significantly decreased plant height and dry biomass. Except MDA and electrolyte leakage, above changes induced by water stress were reversed by H2S synthesis inhibitor aminooxyacetic acid (AOA) and NO synthesis inhibitor NG-nitro-L-arginine methyl ester (L-NAME). However, AOA and L-NAME significantly enhanced MDA production and electrolyte leakage, which further decreased plant height and dry biomass of wheat seedlings under water stress. Application of exogenous H2S donor sodium hydrosulfide (NaHS) to AOA-treated plants and application of exogenous NO donor sodium nitroprusside (SNP) to L-NAME-treated plants reversed above effects of AOA and L-NAME, respectively. Application of LNAME plus water stress significantly decreased NO production induced by water stress. However, application of L-NAME plus water stress had no obvious influence on H2S production induced by water stress, while application of AOA plus water stress significantly reduced the production of H2S and NO induced by water stress. Current findings suggested that H2S acted upstream of NO in the regulation of AsA-GSH cycle in wheat seedlings by water stress. Keywords Hydrogen sulfide . Nitric oxide . Wheat . Ascorbate-glutathione cycle . Water stress
Introduction Water stress has negative effects on plant growth and development, which further reduces plant production. These negative effects are mainly due to the imbalance of water metabolism and oxidative damage induced by water stress (Amrutha et al. 2019; Mohammadi and Moradi 2016). Of above two negative effects, the oxidative damage is largely due to the imbalance of reactive oxygen metabolism induced by water stress. To fight Changjuan Shan and Shang Gao contributed equally to this work. Handling Editor: Néstor Carrillo * Changjuan Shan [email protected] * Hua Li [email protected] 1
Henan Institute of Science and Technology, Xinxiang 453003, China
2
School of Life Sciences, Henan University, Kaifeng 475004, China
against water stress-induced oxidative damage, plants have an efficient antioxidant defense system, which includes enzymatic system and non-enzymatic system (Ghassemi et al. 2019). In plants, ascorbate-glutathione (AsA-GSH) cycle is a very crucial component of the antioxidant defense syste
Data Loading...
