Methyl Jasmonate and Cytokinin Mitigate the Salinity-Induced Oxidative Injury in Wheat Seedlings
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Methyl Jasmonate and Cytokinin Mitigate the Salinity‑Induced Oxidative Injury in Wheat Seedlings Azamat Avalbaev1 · Chulpan Allagulova1 · Dilara Maslennikova1 · Kristina Fedorova1 · Farida Shakirova1 Received: 7 April 2020 / Accepted: 10 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Application of methyl jasmonate (MeJA) has been shown to be effective in improving plant tolerance to various environmental stresses. Present research was conducted to explore the possible role of endogenous cytokinins (CK) in MeJA-mediated alleviation of oxidative stress in wheat plants (Triticum aestivum L.) subjected to sodium chloride salinity. We have compared the effects of 0.1 µM MeJA and 0.04 µM cytokinin 6-benzylaminopurine (BAP), both causing a twofold increase in the level of endogenous cytokinins, on the reactive oxygen species generation, antioxidant enzymes activities, levels of lipid peroxidation, and electrolytes leakage, as well as on the contents of proline and dehydrins under normal and salinity conditions. Salt stress significantly increased generation of superoxide anion and activity of both superoxide dismutase and peroxidase resulting in accelerated lipid peroxidation and electrolyte leakage and also elevated the contents of proline and dehydrins in wheat seedlings. Pretreatment with either MeJA or BAP reduced the salt-induced pro-oxidants/antioxidants imbalance as well as membrane damages. Meanwhile, both hormones promoted additional accumulation of proline and dehydrins under stress and enhanced the barrier properties of the cell walls in seedling roots by accelerating the lignin deposition. Similarity in the protective action of MeJA and cytokinin together with previous data on prevention of the decline in the cytokinin content in MeJA-pretreated salt-stressed wheat seedlings suggest the involvement of endogenous CK in MeJA-induced increase in salinity tolerance of wheat plants. Keywords Methyl jasmonate · Cytokinins · Oxidative stress · Antioxidant enzymes · Dehydrins · Proline Abbreviations MeJA Methyl jasmonate BAP 6-Benzylaminopurine MDA Malondialdehyde ROS Reactive oxygen species SOD Superoxide dismutase CK Cytokinins JA Jasmonic acid
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00344-020-10221-1) contains supplementary material, which is available to authorized users. * Azamat Avalbaev [email protected] 1
Institute of Biochemistry and Genetics ‑ Subdivision of the Ufa Federal Research Centre, Russian Academy of Sciences, pr. Octyabrya, 71, Ufa, Russia 450054
Introduction Jasmonic acid (JA) and its metabolite, methyl jasmonate (MeJA), are endogenous plant growth regulators involved in numerous physiological processes in plants, including seed germination, cell cycle, root growth, leaf senescence, fruit ripening, gravitropism, and reproductive development (Wasternack and Hause 2013; Behr et al. 2018; Ruan et al. 2019). However, jasmonates have received considerable attention due their pivot
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