High-Resolution Imaging of Cellular Proteins Methods and Protocols
This volume presents authoritative and cutting-edge methods and protocols focusing on three tool boxes covering the increasingly diverse methodologies used to image selected proteins and to investigate their function by light and electron microscopy. The
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Steven D. Schwartzbach Omar Skalli Thomas Schikorski Editors
High-Resolution Imaging of Cellular Proteins Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
High-Resolution Imaging of Cellular Proteins Methods and Protocols
Edited by
Steven D. Schwartzbach Department of Biological Sciences, The University of Memphis, Memphis, TN, USA
Omar Skalli Department of Biological Sciences, The University of Memphis, Memphis, TN, USA
Thomas Schikorski Department of Anatomy, Universidad Central del Caribe, Bayamon, PR, USA
Editors Steven D. Schwartzbach Department of Biological Sciences The University of Memphis Memphis, TN, USA
Omar Skalli Department of Biological Sciences The University of Memphis Memphis, TN, USA
Thomas Schikorski Department of Anatomy Universidad Central del Caribe Bayamon, PR, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6350-8 ISBN 978-1-4939-6352-2 (eBook) DOI 10.1007/978-1-4939-6352-2 Library of Congress Control Number: 2016946471 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface Cell biology is the study of the structure and function of cells, cellular organelles, and subcellular structures. Originally, cellular functions were studied without a detailed knowledge of the structures involved and structures were described without an understanding of their function. Imaging techniques that simultaneously studied structure and function or at least correlated function with structure eventually closed this disparity. Several technologies have been greatly responsible for this progress by providing high-resolutio
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