Highly efficient rapid micropropagation and assessment of genetic fidelity of regenerants by ISSR and SCoT markers of So
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ORIGINAL ARTICLE
Highly efficient rapid micropropagation and assessment of genetic fidelity of regenerants by ISSR and SCoT markers of Solanum khasianum Clarke Pavani Chirumamilla1 · Chaitanya Gopu1 · Phanikanth Jogam1 · Shasthree Taduri1 Received: 28 March 2020 / Accepted: 27 October 2020 © Springer Nature B.V. 2020
Abstract An efficient method of rapid micropropagation of Solanum khasianum Clarke was successfully established from the leaf, petiole, and nodal explants. The morphogenetic response of different concentrations of TDZ and BAP individually or in combination with auxins (IAA/IBA/2,4-D) was tested. Friable callus was obtained on different concentrations of BAP alone or in combination with IAA/IBA/2,4-D. Rapid multiple shoot induction was achieved from friable callus on MS medium supplemented with varying concentrations of TDZ and IBA. The leaf explants exhibited a high frequency of multiple shoots than petiole and nodal explants with an optimal percentage of response (92.73%), mean shoot number (53.5 ± 0.47), and shoot length (11.2 ± 0.53 cm) on MS medium augmented with TDZ (1.5 mg l−1) and IBA (1.5 mg l−1). Maximum rooting efficiency was achieved on MS medium with 1.5 mg l−1 IBA with 12.8 ± 0.36 mean number of roots. The in vitro rooted plants were acclimatized with a survival rate of 80%. The genetic fidelity of the regenerants assayed by the ISSR and the SCoT markers showed no genetic variation. The study examined the micropropagation responses of S. khasianum in the presence of various growth regulators and provided a simple and more suitable protocol adapted for the mass propagation of clones in this species. Key message We have established a highly efficient micropropagation system for large scale production in Solanum khasianum. Evaluation of clonal fidelity by using ISSR and SCoT markers detected no somaclonal variations. The present study helps to the enhancement of potential alkaloids (solasodine) with the help of biotechnological tools. Keywords Solanum khasianum · Micropropagation · Genetic fidelity · ISSR · SCoT Abbreviations MS Murashige and Skoogs medium PGRs Plant growth regulators TDZ Thidiazuron BAP Benzylaminopurine IAA Indole acetic acid IBA Indole butyric acid 2,4-D 2,4-Dichlorophenoxy acetic acid Communicated by Silvia Moreno. * Shasthree Taduri [email protected]; [email protected] 1
KN Kinetin NAA 1- Naphthalene acetic acid ANOVA Analysis of variance ISSR Inter simple sequence repeats SCoT Start codon targeted SPSS Statistical package for the social sciences NMR Nuclear magnetic resonance PCR Polymerase chain reaction EDTA Ethylene diamine tetraacetic acid CTAB Cetyl trimethyl ammonium bromide TAE Tris-acetate-EDTA DNA Deoxyribonucleic acid
Plant Cell Tissue and Organ Culture Laboratory, Department of Biotechnology, Kakatiya University, Warangal, Telangana State 506009, India
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Introduction Solanum khasianum Clarke (Solanaceae), commonly known as Kotahi Begena (Regional Research Laboratory, Jorhat), is a perennial
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