Comparative assessment of ISSR, RAPD, and SCoT markers for genetic diversity in Clerodendrum species of North East India
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ORIGINAL ARTICLE
Comparative assessment of ISSR, RAPD, and SCoT markers for genetic diversity in Clerodendrum species of North East India Barbi Gogoi1,2 · S. B. Wann3 · S. P. Saikia1 Received: 25 May 2020 / Accepted: 28 August 2020 © Springer Nature B.V. 2020
Abstract Clerodendrum belonging to the family of Lamiaceae is used in indigenous systems of medicine to treat various life-threatening diseases. The genus has complex morphological variations which lead to limits in its precise taxonomic classifications. Genetic diversity study could enhance taxonomic authentication and evolutionary relationship among the species of Clerodendrum. In this study, nine species of Clerodendrum collected from different regions of North East India were screened using ISSR, RAPD, and SCoT molecular markers. The markers of ISSR, RAPD, and SCoT generated a total of 79, 126, and 145 amplicons with an average of 6.58, 7.86, and 8.53 amplicon per primer. The polymorphism information contents (PIC) for ISSR, RAPD, and SCoT ranged from 0.28 to 0.37, 0.39 to 0.69, and 0.30 to 0.62 with resolving power (Rp) varying from 5.26 to 11.11, 4.04 to 9.67, and 4.54 to 8.65, respectively. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) based clustering methods grouped 94 genotypes into 6 clusters for ISSR and 3 clusters each for RAPD and SCoT markers. Similarly, population structure-based analysis divided 94 genotypes into 6 populations for ISSR and RAPD and 4 populations for SCoT markers. AMOVA analysis revealed that SCoT markers generated maximum genetic variations within and among genotypes, contrary to ISSR and RAPD markers. Results in this study, suggest that the competence of three markers was relatively the same in genotypes fingerprinting, but SCoT was more efficient in the detection of polymorphism for Clerodendrum species. Further, these results could be integrated in the exploration of diverse Clerodendrum species and germplasm utilization. Keywords Clerodendrum · ISSR · RAPD · SCoT · Structure-based
Introduction
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11033-020-05792-x) contains supplementary material, which is available to authorized users. * S. P. Saikia [email protected] S. B. Wann [email protected] 1
Medicinal Aromatic and Economic Plants Group, Biological Sciences & Technology Division (BSTD), CSIR-North East Institute of Science & Technology, Jorhat 785006, Assam, India
2
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India
3
Biotechnology Group, Biological Sciences & Technology Division (BSTD), CSIR-North East Institute of Science & Technology, Jorhat 785006, Assam, India
Clerodendrum L. (Lamiaceae) is a large diversified genus of small trees or shrubs, predominantly distributed in tropical and subtropical regions of Asia, Africa, America, Egypt, and Madagascar. Globally about 580 species were recorded, and 23 species were reported in India, of which 18 species were documented in North East India [1]. The genus had e
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