Human T-Lymphotropic Viruses Methods and Protocols
While HIV-1 continues to be well-researched, this detailed volume draws attention to other members of the Retrovirus family, namely the Human T-lymphotropic Viruses (HTLVs), featuring the most updated technical information about HTLV determination an
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Claudio Casoli Editor
Human T-Lymphotropic Viruses Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Human T-Lymphotropic Viruses Methods and Protocols
Edited by
Claudio Casoli University of Milano, Milano, Italy
Editor Claudio Casoli University of Milano Milano, Italy
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6870-1 ISBN 978-1-4939-6872-5 (eBook) DOI 10.1007/978-1-4939-6872-5 Library of Congress Control Number: 2017933704 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Cover illustration: Two in vitro HTLV-2-infected T lymphocytes stained with anti-Tax-2 polyclonal antibody (yellow). Scale bar= 5 µm. Provided by Dr. Claudio Casoli. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Dedication
With sadness and sorrow, I mourn for Dr. Giovanna Tosi who passed away in July 20, 2016, at the age of 52, after a long illness. Dr. Tosi was assistant professor of immunology at the University of Insubria, Varese. She leaves her old mother Graziella and three sisters, Flavia, Elena, and Adriana. I met Giovanna in 1988 when I came back to Italy after a long stay abroad. She had just obtained the master’s degree in biology at the University of Padova and, as young Ph.D. student, was initiating her research career in Immunology at the University of Verona. It was not difficult to realize soon how sharp was that girl in thinking, planning, and performing science. She rapidly joined my group and she remai
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