IbERF71 , with IbMYB340 and IbbHLH2 , coregulates anthocyanin accumulation by binding to the IbANS1 promoter in purple-f
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ORIGINAL ARTICLE
IbERF71, with IbMYB340 and IbbHLH2, coregulates anthocyanin accumulation by binding to the IbANS1 promoter in purple‑fleshed sweet potato (Ipomoea batatas L.) Zhiyuan Ning1 · Kangdi Hu1 · Zhilin Zhou2 · Donglan Zhao2 · Jun Tang2 · Hong Wang3 · Lixia Li1 · Chen Ding1 · Xiaoyan Chen1 · Gaifang Yao1 · Hua Zhang1 Received: 11 August 2020 / Accepted: 3 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message The transcription factor (TF) IbERF71 forms a novel complex, IbERF71-IbMYB340-IbbHLH2, to coregulate anthocyanin biosynthesis by binding to the IbANS1 promoter in purple-fleshed sweet potatoes. Abstract Purple-fleshed sweet potato (Ipomoea batatas L.) is very popular because of its abundant anthocyanins, which are natural pigments with multiple physiological functions. TFs involved in regulating anthocyanin biosynthesis have been identified in many plants. However, the molecular mechanism of anthocyanin biosynthesis in purple-fleshed sweet potatoes has rarely been examined. In this study, TF IbERF71 and its partners were screened by bioinformatics and RT-qPCR analysis. The results showed that the expression levels of IbERF71 and partners IbMYB340 and IbbHLH2 were higher in purple-fleshed sweet potatoes than in other colors and that the expression levels positively correlated with anthocyanin contents. Moreover, transient expression assays showed that cotransformation of IbMYB340+IbbHLH2 resulted in anthocyanin accumulation in tobacco leaves and strawberry receptacles, and additional IbERF71 significantly increased visual aspects. Furthermore, the combination of the three TFs significantly increased the expression levels of FvANS and FvGST, which are involved in anthocyanin biosynthesis and transport of strawberry receptacles. The dual-luciferase reporter system verified that cotransformation of the three TFs enhanced the transcription activity of IbANS1. In addition, yeast two-hybrid and firefly luciferase complementation assays revealed that IbMYB340 interacted with IbbHLH2 and IbERF71 but IbERF71 could not interact with IbbHLH2 in vitro. In summary, our findings provide novel evidence that IbERF71 and IbMYB340-IbbHLH2 form the regulatory complex IbERF71-IbMYB340-IbbHLH2 that coregulates anthocyanin accumulation by binding to the IbANS1 promoter in purple-fleshed sweet potatoes. Thus, the present study provides a new regulatory network of anthocyanin biosynthesis and strong insight into the color development of purple-fleshed sweet potatoes. Keywords Anthocyanin biosynthesis · Purple-fleshed sweet potato (Ipomoea batatas L.) · IbERF71 · IbMYB340 · Regulatory complex
Communicated by Neal Stewart. Zhiyuan Ning, Kangdi Hu and Zhilin Zhou authors contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00299-020-02621-0) contains supplementary material, which is available to authorized users. * Gaifang Yao [email protected] * Hua Zhang [email protected] Extended author infor
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