Overexpression of the Raphanus sativus RsMYB1 using the flower-specific promoter (InMYB1) enhances anthocyanin accumulat
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Overexpression of the Raphanus sativus RsMYB1 using the flower-specific promoter (InMYB1) enhances anthocyanin accumulation in flowers of transgenic Petunia and their hybrids Aung Htay Naing & Hyun Hee Kang & Hui Yeong Jeong & May Thu Soe & Junping Xu & Chang Kil Kim Received: 3 June 2020 / Accepted: 22 September 2020 / Published online: 29 September 2020 # Springer Nature B.V. 2020
Abstract The generation of transgenic floricultural crops for flower color modification requires a transgene that enhances anthocyanin accumulation and a flowerspecific promoter with stable expression throughout flowering stage. Here, the anthocyanin regulatory R2R3-MYB transcription factor (TF) RsMYB1 was placed under the control of the stable flower-specific promoter (InMYB1) to enhance anthocyanin accumulation in Petunia hybrida cv. Mirage Rose. RsMYB1 overexpression with the flower-specific promoter enhanced anthocyanin accumulation only in floral tissues via the upregulation of key anthocyanin biosynthetic genes. The target gene introgression to another Petunia hybrida cv. White Hawaiian lacking anthocyanins also changed the flower color from white to intense pink in the F1 hybrids by upregulating the biosynthetic genes. Target gene inheritance in the F2 hybrids also produced diverse flower colors, redder than those of non-inheritance F2 hybrids. RsMYB1 overexpression using the flowerspecific promoter (InMYB1) enhanced anthocyanin levels in the petals of transgenic Petunia and its related hybrids. Thus, this system can be used as a promising tool for intensifying the flower color of other
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11032-020-01176-y) contains supplementary material, which is available to authorized users. A. H. Naing : H. H. Kang : H. Y. Jeong : M. T. Soe : J. Xu : C. K. Kim (*) Department of Horticulture, Kyungpook National University, Daegu, Republic of Korea e-mail: [email protected]
commercially important floricultural crops. In addition, the obtained F2MW11 and F2MW12 lines are potential commercial cultivars for the floriculture and landscape industries due to its attractive flower color pattern. Keywords Breeding . Flower color . Genetic transformation . Gene expression
Introduction The floriculture and landscape industries are continuously expanding due to the global demand for ornamental plants, such as cut flowers, potted plants, and bedding plants. Flower shape, color, and scent are the most desirable traits in ornamental plants, so new cultivars with attractive flower colors, forms, and floral scent are highly prized in global markets. Flower color is determined by anthocyanins, flavonoid compounds that produce colors ranging from red to violet and blue (van Tunen and Mol 1991). Thus, flower color can be modified by regulating anthocyanin accumulation in flowering plants. Different approaches, such as conventional breeding, or metabolic genetic engineering, or a combination of both, have been used to develop a large number of flowering plants with nove
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