Identification and full-genome sequencing of canine kobuvirus in canine fecal samples collected from Anhui Province, eas
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ORIGINAL ARTICLE
Identification and full‑genome sequencing of canine kobuvirus in canine fecal samples collected from Anhui Province, eastern China Yong Wang1 · Yongqiu Cui1 · Yeqiu Li1 · Xiaopeng Wang1 · Kankan Yang1 · Da Zhang1 · Liang Zhao1 · Caixia Bai1 · Shudong Jiang1 · Yongdong Li2 Received: 9 January 2020 / Accepted: 9 July 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract Canine kobuvirus (CaKoV), a newly described virus, is the causative agent of gastroenteritis in dogs. In this study, 57 fecal samples from dogs with diarrhea in Anhui Province, eastern China, were collected. Among these, five samples were identified to be infected with CaKoV, by polymerase chain reaction targeting the CaKoV 3D gene. The five CaKoV strains were subjected to phylogenetic analysis. The sequences of VP1 from the five CaKoV strains were 93.6%–96.1% identical to each other and 91.75%–97.95% identical to other reported CaKoV VP1 sequences. In addition, the complete genome of one strain was successfully amplified and sequenced. The genome consisted of 8223 nucleotides and shared 94.6%–97.0% nucleotide and 93.1%–94.0% amino acid sequence identity with other CaKoV isolates. Phylogenetic analysis revealed that the CaKoV strain from Anhui Province was similar to other Chinese strains, and it was more closely related to feline and mouse kobuviruses than to sheep and bovine kobuviruses. Interestingly, all of the CaKoV-positive samples were coinfected with canine parvovirus. The finding of CaKoV infection in dogs with diarrhea and coinfection with canine parvovirus are a cause for concern and highlight the need for management and preventive measures.
Introduction Kobuvirus, a genus of the family Picornaviridae, is divided into six species, namely Aichivirus A (human kobuvirus), Aichivirus B (bovine kobuvirus), Aichivirus C (porcine kobuvirus), Aichivirus D (kagovirus 1), Aichivirus E (rabbit picornavirus), and Aichivirus F (bat kobuvirus) [3, 16, 20, 21]. Canine kobuvirus (CaKoV) is a member of the species Handling Editor: Tim Skern. Yong Wang and Yongqiu Cui contributed equally to this work and considered to be the co-first authors. * Shudong Jiang [email protected] * Yongdong Li [email protected] 1
Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, People’s Republic of China
Municipal Key Laboratory of Virology, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China
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Aichivirus A [1, 9]. In 2011, the whole genome sequence of a CaKoV isolate was first determined in the United States of America [9]. Thereafter, the complete genomes of CaKoV isolates have been sequenced in various countries, including China, Britain, Italy, South Korea, Tanzania, Kenya, and Japan [2, 6, 7, 10, 14, 19]. The CaKoV genome is 8.1–8.2 kb long, with one open reading frame (ORF) and a 5’ untranslated region (UTR). The ORF contains 7332–7341 nucleotides (nt) that encode a polyprotein of
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