Immunoelectron Microscopy Methods and Protocols
Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protoc
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MO L E C U L A R BI O L O G Y
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For other titles published in this series, go to www.springer.com/series/7651
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Immunoelectron Microscopy Methods and Protocols
Edited by
Steven D. Schwartzbach Department of Biology, University of Memphis, Memphis, TN, USA
Tetsuaki Osafune Department of Life Science, Nippon Sport Science University, Yokohama, Japan
Editors Steven D. Schwartzbach Department of Biology University of Memphis 3774 Walker Avenue Memphis, TN 38152 USA [email protected]
Tetsuaki Osafune Department of Life Science Nippon Sport Science University Kamosida 1221-1 227-0033 Yokohama Japan [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60761-782-2 e-ISBN 978-1-60761-783-9 DOI 10.1007/978-1-60761-783-9 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2010929610 © Springer Science+Business Media, LLC 2010 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Cover illustration: A 3D solid surface rendering showing that the immunogold-labeled small subunit of ribulose-bisphosphate carboxylase, yellow dots, is concentrated in the propyrenoid, blue, rather than being uniformly distributed throughout the prolamellar body, red, and proplastid. The 3D reconstruction of the distribution of ribulose-bisphosphate carboxylase is shown in the left, while the 3D reconstruction of the propyrenoid and prolamellar body superimposed upon the 3D distribution of ribulose-bis-phosphate carboxylase is shown in the right. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Cell biology is the science of correlating cell structure and function. The electron microscopist obtains high-resolution pictures of the intricate structures found in cells. Electron tomography and serial sections can be used to reconstruct the three-dimensional structure of the cell and its organelles. Pictures are worth a thousand words, but they are unable to provide information regarding the function of the intricate structures found in cells and their macromolecular composition. The biochemist and molecular biologist determine the functions of the molecules, macromolecular complexes, and organelles found within cells. They isolate individual
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