Light Microscopy Methods and Protocols
This volume addresses up-to-date light microscopy approaches and toolsets offered for live- or fixed-cell observations. The imaging strategies discussed in this book include confocal laser scanning and spinning disk confocal microscopy, FRET, FRAP, and la
- PDF / 12,110,355 Bytes
- 283 Pages / 504.63 x 737.01 pts Page_size
- 90 Downloads / 194 Views
Yolanda Markaki Hartmann Harz Editors
Light Microscopy Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Light Microscopy Methods and Protocols
Edited by
Yolanda Markaki UCLA School of Medicine, Department of Biological Chemistry, Los Angeles, CA, USA
Hartmann Harz Biozentrum der LMU Munchen, Planegg-Martinsried, Germany
Editors Yolanda Markaki UCLA School of Medicine Department of Biological Chemistry Los Angeles, CA, USA
Hartmann Harz Biozentrum der LMU Munchen Planegg-Martinsried, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6808-4 ISBN 978-1-4939-6810-7 (eBook) DOI 10.1007/978-1-4939-6810-7 Library of Congress Control Number: 2017931658 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Cover image: Photoactivated localization microscopy (PALM) detecting a bacterial membrane protein. Shown is a Bacillus subtilis cell expressing FloA-mNeonGreen. FloA is a bacterial flotillin-like protein, involved in membrane compartmentalization. PALM images were acquired in TIRF. Detected signals were filtered for PSF width (100–200 nm) and photon count (200–1000 photons). The average localization precision of detected FloA-mNeonGreen molecules is 25 nm. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Recent achievements, such as the development of a new generation of nanoscopes surpassing the Abbe’s diffraction limit or high-resolution approaches for deep imaging, such as light-sheet or
Data Loading...
