Light Microscopy Methods and Protocols
Of all scientific instruments, probably none has had more applications in the life sciences than the light microscope. In Light Microscopy: Methods and Protocols, expert researchers explore the basics and the latest advances in microscope instrumentation,
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MO L E C U L A R BI O L O G Y
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For other titles published in this series, go to www.springer.com/series/7651
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Light Microscopy Methods and Protocols
Edited by
Hélio Chiarini-Garcia Laboratory of Structural Biology and Reproduction, Department of Morphology, ICB, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil
Rossana C.N. Melo Laboratory of Cellular Biology, Department of Biology, ICB, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil
Editors Hélio Chiarini-Garcia Department of Morphology Federal University of Minas Gerais Belo Horizonte, MG 31270-901, Brazil [email protected]
Rossana C.N. Melo Department of Biology Federal University of Juiz de Fora Juiz de Fora, MG 36036-900, Brazil [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60761-949-9 e-ISBN 978-1-60761-950-5 DOI 10.1007/978-1-60761-950-5 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2010936902 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Of all scientific instruments, probably none has had more applications in the life sciences than the light microscope. Advances in microscope instrumentation, sample preparation and imaging techniques have been producing fundamental insights into the functions of cells and tissues. The protocols in Light Microscopy: Methods and Protocols cover a variety of brightfield and fluorescence microscopy-based approaches central to the study of a range of biological questions. The book provides information on how to prepare cells and tissues for microscopic investigations, including detailed staining procedures and how to analyze images and interpret results accurately. Techniques are presented in a friendly, step-by-step fashion with helpful information and useful tips. Section I covers selected applications of bright-field microscopy to the study of animal and plant biology. Section II covers the fundamental principles of fluorescence microscopy as well as its applications to multiple fields including immunology, ecology, cancer biology and cell signaling. Light Microsco
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