Super-Resolution Microscopy Methods and Protocols
This volume presents current advanced technologies and methods used in super-resolution microscopy. The chapters in this book cover a wide range of topics such as introducing super-resolution microscopy into a core facility; two-photon STED microscopy for
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Holger Erfle Editor
SuperResolution Microscopy Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences, University of Hertfordshire, Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Super-Resolution Microscopy Methods and Protocols
Edited by
Holger Erfle High-Content Analysis of the Cell (HiCell) and Advanced Biological Screening Facility, BioQuant, Heidelberg University, Heidelberg, Germany
Editor Holger Erfle High-Content Analysis of the Cell (HiCell) and Advanced Biological Screening Facility BioQuant, Heidelberg University Heidelberg, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7264-7 ISBN 978-1-4939-7265-4 (eBook) DOI 10.1007/978-1-4939-7265-4 Library of Congress Control Number: 2017950493 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Super-resolution microscopy also known as nanoscopy opens a new avenue in the success story of fluorescence microscopy in modern biology by surpassing Abbe’s diffraction limit. It bridges the resolution gap to electron microscopy by resolving structures at the low nanometer scale. In addition, it maintains the advantages of optical fluorescence microscopy like simple sample preparation allowing breakthrough discoveries in diverse directions of life sciences. This book aims to provide a current overview of the advanced technologies and methods in super-resolution microscopy addressing novices as well as experienced users in this field. We start with a mini-review, followed by a ma
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