Improved Methane Production Using Lignocellulolytic Enzymes from Trichoderma koningiopsis TM3 Through Co-digestion of Pa
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ORIGINAL PAPER
Improved Methane Production Using Lignocellulolytic Enzymes from Trichoderma koningiopsis TM3 Through Co‑digestion of Palm Oil Mill Effluent and Oil Palm Trunk Residues Tanawut Nutongkaew1 · Poonsuk Prasertsan2 · Sompong O‑Thong3 · Sukonlarat Chanthong1 · Wasana Suyotha1 Received: 20 April 2019 / Accepted: 3 October 2019 © Springer Nature B.V. 2019
Abstract The efficacy of concentrated enzymes from Trichoderma koningiopsis TM3 in hydrolyzing palm oil mill effluent (POME) and oil palm trunk residues (OPTr) at 40 and 50 °C was evaluated prior to methane fermentation. POME hydrolysate containing total sugar concentration of 15.40 g L −1 was obtained from enzymatic hydrolysis using 15 Unit g −1 TVS at 50 °C for 18 h incubation with the hydrolysis yield of 0.35 g total sugars g−1 TVS. The OPTr hydrolysate contained slightly higher total sugar concentration (18.90 g L−1) with the hydrolysis yield of 0.85 g total sugars g −1 TVS under the same condition. Methane production from POME hydrolysate was 6.29% higher than the raw POME. Co-digestion of POME hydrolysate with OPTr gave the maximum methane yield (369 ml CH4 g−1 VS-added) with the increase of 9.28% compared to the raw POME. The methane production rate (Rmax) and the hydrolysis rate constant (kh) of the co-digestion of POME hydrolysate with OPTr were 1.2-fold higher than those of the POME hydrolysate. PCR-DGGE analysis revealed that Clostridium sp. and Petrimonas sp. were dominated bacteria while Methanosarcina sp. and Methanospirillum sp. played an important role in methane production. These results indicated that enzymatic pretreatment and co-digestion of POME hydrolysate with OPTr could improve methane yield from anaerobic fermentation of POME. Graphic Abstract
Keywords Enzymatic hydrolysis · Methane production · Co-digestion · Palm oil mill effluent · Oil palm trunk residues · Microbial community Extended author information available on the last page of the article
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Statement of Novelty This research work initiated the valorization of POME and OPT residues (OPTr) through enzymatic hydrolysis and co-digestion process to increase methane yield in biogas production from POME. Enzymes produced by our newly isolated strain Trichoderma koningiopsis TM3 was employed to hydrolyze the POME and resulted in higher methane yield. Further increase the methane yield was achieved through the co-digestion of POME hydrolysate with OPTr.This is the first report to illustrate the enhancement of biogas production from POME by combination of enzymatic hydrolysis and co-digestion with OPTr and comparison on the digestion kinetics parameters of singleand co-digestion of POME/POME hydrolysate with OPTr/ OPTr hydrolysate in an anaerobic digestion system using the modified Gompertz equation.
Introduction In Southeast Asia, particularly in Malaysia, Indonesia and Thailand, oil palm is one of the most important commercial crops. The process to extract the oil generates significantly large quantities of wastewater or palm oil mill effluen
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