In Situ Hybridization Protocols
In Situ Hybridization Protocols, Fourth Edition, contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules. Written in the highly successful Methods in Molecu
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Boye Schnack Nielsen Editor
In Situ Hybridization Protocols Fourth Edition
METHODS
IN
M O L E C U L A R B I O LO G Y
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
In Situ Hybridization Protocols Fourth Edition
Edited by
Boye Schnack Nielsen Bioneer A/S, Molecular Histology, Hørsholm, Denmark
Editor Boye Schnack Nielsen Bioneer A/S Molecular Histology Hørsholm, Denmark
ISSN 1064-3745 ISSN 1940-6029 (electronic) ISBN 978-1-4939-1458-6 ISBN 978-1-4939-1459-3 (eBook) DOI 10.1007/978-1-4939-1459-3 Springer New York Heidelberg Dordrecht London Library of Congress Control Number: 2014944535 © Springer Science+Business Media New York 2014 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. Exempted from this legal reservation are brief excerpts in connection with reviews or scholarly analysis or material supplied specifically for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work. Duplication of this publication or parts thereof is permitted only under the provisions of the Copyright Law of the Publisher’s location, in its current version, and permission for use must always be obtained from Springer. Permissions for use may be obtained through RightsLink at the Copyright Clearance Center. Violations are liable to prosecution under the respective Copyright Law. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. While the advice and information in this book are believed to be true and accurate at the date of publication, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Humana Press is a brand of Springer Springer is part of Springer Science+Business Media (www.springer.com)
Preface Ribonucleic acid (RNA) molecules are fragile single-stranded transcripts of genetic DNA, generally considered as the coding messenger needed to produce protein from the DNA. These RNA messengers (mRNA) have together with transfer RNA (tRNA) and ribosomal RNA (rRNA) historically been considered the essentials in RNA biology, driving the translation of RNA into protein. Today it is known that only a minor pe
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