In Situ Hybridization
Leading researchers present contemporary treatment of in situ hybridization applied to current issues in animal virus pathogenesis. The most recent methods are given for locating viral genes in whole animal section and for defining the number and type of
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Editors R. W. Compans, Birmingham!Alabama . M. Cooper,
Birmingham!Alabama . H. Koprowski, Philadelphia I. McConell, Edinburgh . F. Melchers, Basel V. Nussenzweig, NewYork . M.Oldstone, La Jolla/California . S. Olsnes, Oslo . H. Saedler, Cologne . P. K Vogt, Los Angeles . H. Wagner, VIm I. Wilson, La Jolla/California
In Situ Hybridization Edited by A. T. Haase and M. B. A. Oldstone
With 33 Figures, 8 in colored
Springer-Verlag Berlin Heidelberg NewYork London Paris Tokyo Hong Kong
Ashley T. Haase, M. D. Department of Microbiology University of Minnesota Medical Scool Minneapolis, MN 55455, USA
Michael B. A. Oldstone, M. D. Department of Immunology Scripps Clinic and Research Foundation 10666 North Torrey Pines Rd., La Jolla, CA 92037, USA
ISBN-13 :978-3-642-74427-3 DOl: 10.1007/978-3-642-74425-9
e-ISBN-13:978-3-642-74425-9
This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in other ways, and storage in data banks. Duplication of this publication or parts thereof is only permitted under the provisions of the German Copyright Law of September 9, 1965, in its version of June 24, 1985, and a copyright fee must always be paid. Violations fall under the prosecution act of the German Copyright Law.
© Springer-Verlag Berlin Heidelberg 1989 Softcover reprint of the hardcover 1st edition 1989 Library of Congress Catalog Card Number 15-12910 The use of registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Product Liability: The publisher can give no guarantee for information about drug dosage and application thereof contained on this book. In every individual case the respective user must check its accuracy by consulting other pharmaceutical literature. 2123/3020-543210 - Printed on acid-free paper
Foreword
The ability to locate viral genes, transcripts and proteins to unique cells and tissues in vivo while preserving morphology has been a powerful tool for understanding pathogenesis. Because of the several ingenious ways in situ hybridization Can be used, and in consideration of some difficulties with the technology, this volume was conceived to address both of these issues. Ashley Haase, who has been one of the leaders in developing and popularizing the utility of this approach for the study of pathogenesis, has admirably put together this .volume with these views in mind. Michael B. A. Oldstone Research Institute of Scripps Clinic
Preface
Viral infections can be conceptually characterized as a series of discrete events, beginning with invasion of the host and continuing with multiplication at the site of entry and subsequent dissemination throughout the organism. The fate of the virus (or the host, depending on one's viewpoint), is determine
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