In vitro and in vivo treatments of Echinococcus granulosus with Huaier aqueous extract and albendazole liposome
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ORIGINAL PAPER
In vitro and in vivo treatments of Echinococcus granulosus with Huaier aqueous extract and albendazole liposome Hailong Lv & Yufeng Jiang & Min Liao & Hongli Sun & Shijie Zhang & Xinyu Peng
Received: 29 November 2011 / Accepted: 12 September 2012 / Published online: 23 September 2012 # Springer-Verlag Berlin Heidelberg 2012
Abstract The aim of this study was to investigate the in vitro and in vivo efficacies of chemotherapy employing albendazole liposome (L-ABZ), Huaier aqueous extract, and a Huaier aqueous extract/L-ABZ combination against Echinococcus granulosus. Protoscolices of E. granulosus were incubated in vitro with the two drugs, either separately or in combination, at the following final concentrations: 2 mg/mL Huaier aqueous extract, 10 μg/mL L-ABZ, and 2 mg/mL Huaier aqueous extract + 10 μg/mLL-ABZ. Huaier aqueous extract and L-ABZ displayed slower protoscolicidal activity when applied separately than when used in combination. The maximum protoscolicidal effect was found with the combination Huaier aqueous extract + LABZ. Despite the low Huaier aqueous extract + L-ABZ concentrations used, protoscolex viability dropped rapidly. Hailong Lv and Yufeng Jiang contribute equally to the manuscript. H. Lv : S. Zhang : X. Peng The Hepatic Surgery Center, Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China H. Lv : S. Zhang : X. Peng (*) Department of Hepatobiliary Surgery, The First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi 832008, Xinjiang, China e-mail: [email protected] Y. Jiang (*) Department of Histology and Embryology, School of Medicine, Shihezi University, Shihezi 832008, Xinjiang, China e-mail: [email protected] M. Liao Department of Histology and Embryology, Wenzhou Medical College, Wenzhou 325000, Zhejiang, China H. Sun Department of Hematology, Wuxi People’s Hospital, Wuxi 214023, Zhejiang, China
In vivo studies were performed on mice injected with protoscolices of E. granulosus. Huaier aqueous extract and LABZ were administered three times a week for a period of 4 months by the oral route. Huaier aqueous extract in E. granulosus-infected mice was effective. Combined application of both drugs did increase the treatment efficacy. In conclusion, the outcomes obtained clearly demonstrated that in vitro and in vivo treatment with Huaier aqueous extract and L-ABZ is effective against E. granulosus.
Introduction Cystic echinococcosis (CE) caused by the larval stage of Echinococcus granulosus is a serious, life-threatening public health problem. The disease is distributed worldwide, and affects humans as well as domestic livestock including cattle, sheep, horses, and others (Eckert and Deplazes 2004). The definitive host is the dog, in which adult tapeworms attach to the intestinal epithelium and undergo sexual reproduction, leading to the development of eggs. These eggs are shed into the environment with the feces. The eggs contain an oncosphere, which upon ingestion by a suitable intermediate h
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