In vitro evaluation of a novel multiwalled carbon nanotube/nanohydroxyapatite/polycaprolactone composite for bone tissue
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In vitro evaluation of a novel multiwalled carbon nanotube/nanohydroxyapatite/polycaprolactone composite for bone tissue engineering Huixiao Yang1,b), Jieqing Li2,b), Qiong Liao3, Hua Guo4, Huishan Chen1, Yuting Zhu1, Meijuan Cai1, Huling Lv5,a) 1
Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatological Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510140, People’s Republic of China Department of Breast Surgery, Tianjin Central Hospital of Gynecology and Obstetrics, Tianjin 300100, People’s Republic of China; and Division of Cancer Research and Training, Department of Internal Medicine, Charles R. Drew University of Medicine and Science, Los Angeles, California 90059, USA 3 Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education (Sichuan University) China, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, People’s Republic of China 4 Shriners Hospitals Pediatric Research Center, Lewis Katz School of Medicine, Temple University, Philadelphia, Pennsylvania 19140, USA 5 Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatological Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510140, People’s Republic of China; and State Key Laboratory of Oral Diseases, Sichuan University West China College of Stomatology, Sichuan University, Chengdu, Sichuan 610041, People’s Republic of China a) Address all correspondence to this author. e-mail: [email protected] b) These authors contributed equally to this work. 2
Received: 14 May 2018; accepted: 6 December 2018
In this study, a three-phased multiwalled scaffold, composed of carbon nanotube (mwCNT), nanocrystalline hydroxyapatite (nHA), and polycaprolactone (PCL), was fabricated by the solvent evaporation technique. The structure character, mechanical properties, and degradation activity in simulated body fluid (SBF), along with osteoproductive ability in human osteosarcoma cell MG63, were investigated thoroughly. Results showed that the three phases in mwCNT/nHA/PCL composite presented excellent miscibility and stronger interfacial force when the weight content was 1/15/84 (wt%). Simultaneously, the composite had smaller porosity and slower degradation rate, and there was massive crystallized hydroxyapatite formed on the surface after being soaked in SBF. With regard to bioactivity, MG63s on this scaffolds presented good proliferation performance and differentiated into the osteogenic lineage by expressing high levels of ALP. It was concluded that mwCNTs/nHA/PCL composite scaffolds might be beneficial for bone tissue engineering at a relatively low concentration of mwCNTs and nHA.
Introduction Massive bone defects caused by infection, tumor, abnormalities, and trauma need treatments to facilitate their repair, replacement, or regeneration. The developing field of tissue engineering (TE) aims to regenerate damaged tissues by combining cells from the body with highly porous scaffold biomaterials, which act as templates fo
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