In Vitro Evaluation of the Antibacterial Properties of Tea Tree Oil on Planktonic and Biofilm-Forming Streptococcus muta
- PDF / 4,602,295 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 47 Downloads / 196 Views
Research Article Theme: Formulation and Delivery of Natural Products Guest Editors: Harsh Chauhan, Abhijit Date and Sonali Dhindwal
In Vitro Evaluation of the Antibacterial Properties of Tea Tree Oil on Planktonic and Biofilm-Forming Streptococcus mutans Yu-Meng Song,1 Hong-Yan Zhou,1,2 Ye Wu,3 Jue Wang,1,2 Qian Liu,1,2 and Yu-Feng Mei1,2,4
Received 23 March 2020; accepted 9 July 2020 Abstract. Streptococcus mutans (S. mutans) is the principal etiologic agent in the occurrence of human dental caries and the formation of biofilms on the surface of teeth. Tea tree oil (TTO) has been demonstrated to exhibit a wide range of pharmacological actions that can effectively inhibit the activity of bacteria. In this context, we evaluated the in vitro antimicrobial effects of TTO on S. mutans both during planktonic growth and in biofilms compared with 0.2% CHX. We determined the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) using the microdilution method, the bacteriostatic rate using an MTT assay, and the antimicrobial time using a time–kill assay. Then, we explored the effects of TTO on acid production and cell integrity. Furthermore, the effects of TTO on the biomass and bacterial activity of S. mutans biofilms were studied. Finally, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) were used to investigate the structure and activity of biofilms. The MIC and MBC values were 0.125% and 0.25%, and the bacterial inhibition rate was concentration dependent. TTO can effectively inhibit bacterial acid production and destroy the integrity of the cell membrane. Electron micrographs revealed a reduction in bacterial aggregation, inhibited biofilm formation, and reduced biofilm thickness. The effect of TTO was the same as that of 0.2% CHX at a specific concentration. In summary, we suggest that TTO is a potential anticariogenic agent that can be used against S. mutans. KEY WORDS: Tea tree oil; Streptococcus mutans; Biofilm; Antimicrobial activities.
INTRODUCTION Dental caries is one of the most widespread medical conditions and is caused by a mixture of microorganisms and food debris. Although there are billions of microbial cells in the cavity, scientific studies have shown that Streptococcus mutans is most responsible for the occurrence of dental caries (1). S. mutans is an active acid producer and can adhere to the enamel salivary pellicle and to other plaque bacteria to form biofilms. The biofilm affords many benefits to bacteria and Yu-Meng Song and Hong-Yan Zhou are authors contributed equally to the work and share the first authorship. Guest Editors: Harsh Chauhan, Abhijit Date and Sonali Dhindwal 1
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, 210029, China. 2 Department of Pediatric Dentistry, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, China. 3 Department of Stomatology Nanjing First Hospital Nanjing Medical University, Nanjing, China. 4 To whom correspondence should be addressed. (e–mail: yfmei@nj
Data Loading...
