In vitro bioactivity and antibacterial properties of bismuth oxide modified bioactive glasses
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ishree Ratha Bioceramics and Coating Division, CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India
Tarun Adarsh Ram Lal Anand College, Delhi University, New Delhi-110 021, India
Akrity Anand Bioceramics and Coating Division, CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India
Prasanta Kumar Sinha Academy of Scientific and Innovative Research (AcSIR), Campus: CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India; and Materials Characterization & Instrumentation Division, CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India
Prerna Diwan Ram Lal Anand College, Delhi University, New Delhi-110 021, India
Kalyandurg Annapurna and Kaushik Biswasa) Academy of Scientific and Innovative Research (AcSIR), Campus: CSIR-Central Glass & Ceramic Research Institute, Kolkata-700 032, India; and Glass Division, CSIR-Central Glass & Ceramic Research Institute, Kolkata700 032, India (Received 6 September 2017; accepted 8 November 2017)
Chronic osteomyelitis, a bone infection caused by bacteria, requires extensive parenteral treatments. With an aim to develop bioactive glass with antibacterial properties to resist such infections, bioactive glasses with bismuth oxide as the dopant in various amounts up to 8 wt% were prepared. X-ray diffraction patterns and Fourier-transform infrared spectra of glass samples after immersion in simulated body fluid showed the presence of hydroxyapatite (HAp) and hydroxyl carbonate apatite for all samples except with the one having Bi2O3 substitution of 8 wt%. In vitro cell proliferation by MTT assay studies using a mouse fibroblast cell line (NIH3T3) have also been carried out. Primary antimicrobial activity of the glass particles was analyzed against Escherichia coli (E. coli) using broth microdilution method which exhibited bacteriostatic effects and bactericidal properties in selected samples. The combination of bioactivity, cell proliferation, and antibacterial properties of selected Bismuth-containing bioactive glasses could be exploited in treating bone-related infections.
I. INTRODUCTION
Bioactive glasses have a wide range of clinical applications in the field of orthopedics for the repair and regeneration of bone, dentistry for teeth repair, and treatment against diseases like osteoporosis and osteomyelitis.1 Bioactive glasses bond to bone due to their ability to form an interface that helps in biological fixation with the natural bone.2 The interface is formed when the surface of the bioactive glass interacts with the Contributing Editor: Amit Bandyopadhyay a) Address all correspondence to this author. e-mail: [email protected] DOI: 10.1557/jmr.2017.442
physiological environment inside the body and is found to be made of biologically active hydroxyl carbonate apatite (HCA), which is chemically and structurally similar to the apatite mineral of natural bone.2 Hench developed first bioactive glass in SiO2–Na2O–CaO–P2O5 system known as 45S5 (BioglassÒ) which has been commercially used for many bone-related treatments. O
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