In vitro investigation of chemical properties and biocompatibility of neurovascular braided implants
- PDF / 2,798,384 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 113 Downloads / 216 Views
BIOCOMPATIBILITY STUDIES Original Research
In vitro investigation of chemical properties and biocompatibility of neurovascular braided implants Giorgio Cattaneo1 Chris Bräuner2 Gerd Siekmeyer3 Andreas Ding1 Sabina Bauer1 Markus Wohlschlögel2 Lisa Lang4 Teresa Hierlemann4 Maria Akimov4 Christian Schlensak4 Andreas Schüßler1 Hans-Peter Wendel4 Stefanie Krajewski4 ●
●
●
●
●
●
●
●
●
●
●
●
1234567890();,:
1234567890();,:
Received: 21 September 2018 / Revised: 6 May 2019 / Accepted: 18 May 2019 / Published online: 4 June 2019 © Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract Braiding of Nitinol micro wires is an established technology for the manufacturing of fine-meshed neurovascular implants for tortuous vessel geometries. Electropolishing of wires before the braiding process has the potential to improve the in vitro behaviour in terms of thrombogenicity and endothelial cell proliferation. In this study, we present the first in vitro investigation of braided electropolished/blue oxide Nitinol samples in a blood flow loop, showing a significantly lower activation of the coagulation pathway (represented by the TAT III marker) and a tendency towards reduced platelet adhesion. Furthermore, we applied the same surface treatment on flat disks and measured protein adhesion as well as endothelial cell proliferation. We compared our results to non-electropolished samples with a native oxide surface. While platelet deposition was reduced on electropolished/blue oxide surface, a significant increase of endothelial cell seeding was observed. Investigation of inflammatory marker expression in endothelial cells provided divergent results depending on the marker tested, demanding closer investigation. Surface analysis using Auger electron spectroscopy revealed a thin layer mainly consisting of titanium oxynitride or titanium oxide + titanium nitride as a potential cause of the improved biological performance. Translated to the clinical field of intracranial aneurysm treatment, the improved biocompatibility has the potential to increase both safety (low thrombogenicity) and effectiveness (aneurysm neck reconstruction).
Supplementary information The online version of this article (https:// doi.org/10.1007/s10856-019-6270-6) contains supplementary material, which is available to authorized users. * Giorgio Cattaneo [email protected] 1
Acandis GmbH, Pforzheim, Germany
2
Admedes GmbH, Pforzheim, Germany
3
Acquandas GmbH, Kiel, Germany
4
Department of Thoracic and Cardiovascular Surgery, Clinical Research Laboratory, University Medical Center, Tuebingen, Germany
67
Page 2 of 12
Journal of Materials Science: Materials in Medicine (2019) 30:67
Graphical Abstract
1 Introduction In the last three decades, braided stents have become established devices for endovascular treatment of blood vessel disease. Starting in 1986 with the introduction of the Wallstent™ endoprosthesis for treatment of coronary artery stenosis [1], braided stents nowadays find broad application in the treatment of caroti
Data Loading...
