In vitro and in vivo biocompatibility of polyurethanes synthesized with castor oil polyols for biomedical devices
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In vitro and in vivo biocompatibility of polyurethanes synthesized with castor oil polyols for biomedical devices Yomaira L. Uscátegui1, Luis E. Díaz2, Manuel F. Valero3,a) 1
Doctoral Program in Bioscience, Research Group on Energy, Materials and Environment, Universidad de La Sabana, Chía, Cundinamarca 53753, Colombia 2 Research Group on Bioprospecting, Universidad de La Sabana, Chía, Cundinamarca 53753, Colombia 3 Chemical Engineering Program, Research Group on Energy, Materials and Environment, Universidad de La Sabana, Chía, Cundinamarca 53753, Colombia a) Address all correspondence to this author. e-mail: [email protected] Received: 20 April 2018; accepted: 8 November 2018
Polyurethanes (PUs) were synthesized with polyols derived from castor oil and isophorone diisocyanate. The materials were evaluated for their mechanical properties using stress–strain curves, thermogravimetric analysis, differential scanning calorimetry, and contact angle analysis. The biological response of the materials was evaluated by determining their cell viability in vitro, antimicrobial activity against Escherichia coli and Pseudomonas aeruginosa, and biological response in vivo of PUs by means of implanting them in Wistar rats. The cell proliferation on the materials was analyzed using mouse fibroblast L929, human fibroblast MRC-5, and adult human dermal fibroblast (HDFa) cells by the ISO 10993-5 method. The materials showed no toxic effects and promoted cell proliferation. Experiments performed in vivo for 30 days in mice showed that the materials neither affected the wound healing process nor caused adverse effects or severe injuries in the dorsal midcervical tissue or organs on histological evaluation. PUs synthesized can be used in biomedical devices.
Introduction One drawback of using wound closure devices is the high tension the materials produced on the wound during healing. This high tension has been observed to generate necrosis, destroy the tissue, and promote eversion or inversion of wound edges [1, 2]. The friction performance of wound closure devices plays a vital role in their functionality. In general, the friction generated between wound closure devices and tissue can cause inflammation, pain, and increased recovery time [3]. One possible solution proposed by Vogels et al. is to use materials with the ability to regulate the tension through elasticity, improving the wound healing process. Therefore, in this work, PUs are examined as a candidate material for the use in wound closure devices because of their various desirable properties [1]. Therefore, the selection of monomers and additives for the synthesis of polyurethanes (PUs) is essential to determine the final properties and therefore the desired application [4]. At present, synthetic polymers signify a great development in the design of biomaterials for different applications because of their use in supports for tissue engineering, controlled
ª Materials Research Society 2019
release systems, implants, artificial skin, devices for osteosynthesis, and als
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