In Vitro Mutagenesis Protocols Third Edition
In the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions of components of the proteome. The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
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In Vitro Mutagenesis Protocols Third Edition
Edited by
Jeff Braman Stratagene, An Agilent Technologies Division, La Jolla, CA, USA
Editor Jeff Braman, Ph.D. Stratagene An Agilent Technologies Division La Jolla, CA USA [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60761-651-1 e-ISBN 978-1-60761-652-8 DOI 10.1007/978-1-60761-652-8 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2010925108 © Springer Science+Business Media, LLC 2010 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Cover illustration: Based on Figure 2A of Chapter 16 Printed on acid-free paper Humana Press is a part of Springer Science+Business Media (www.springer.com)
Preface In the preface to the second edition of this volume, I claimed that the book represented a toolbox containing protocols to advance the understanding of the connection between nucleotide sequence and sequence function. The same holds true in this third edition, with a notable exception; the third edition contains a variety of specialty tools successfully employed by scientists just like you to unravel the intricacies of protein–protein interaction, protein structure–function, protein regulation of biological processes, and protein activity. A novel section is included containing mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. Each chapter was expanded from the “Methods” section of a paper published in a reputable peer-reviewed journal for the purpose of solving one or more of the problems described above. Chapter “Notes” are included to highlight critical experimental details. Many of the authors describe the utility of their protocol to answer a difficult experimental question. All th
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