In Vitro Mutagenesis Methods and Protocols
In vitro mutagenesis remains a critical experimental approach for investigating gene and protein function at the cellular level. This volume provides a wide variety of updated and novel approaches for performing in vitro mutagenesis using such m
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Andrew Reeves Editor
In Vitro Mutagenesis Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
In Vitro Mutagenesis Methods and Protocols
Edited by
Andrew Reeves Coskata, Inc., Warrenville, IL, USA
Editor Andrew Reeves Coskata, Inc. Warrenville, IL, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6470-3 ISBN 978-1-4939-6472-7 (eBook) DOI 10.1007/978-1-4939-6472-7 Library of Congress Control Number: 2016954797 © Springer Science+Business Media New York 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface In vitro mutagenesis remains an essential tool for molecular biologists, biochemists, and metabolic engineers in performing both basic and applied research into gene and protein function. It is a cross-disciplinary research and development approach that has broad applications in many important fields. With the advent of ever more sophisticated investigative and analytical methods such as next-generation sequencing, emerging, powerful geneediting techniques, and comprehensive data analysis software programs to process large data sets, the molecular biology field is witnessing a magnitude change in the understanding of gene and protein structural and functional relationships at the organismal level. With these powerful tools at researchers’ disposal, it is now conceivable to design an in vitro mutagenesis strategy in a genetically manipulable organism, carry out the mutagenesis experiments in an efficient manner, generate, retrieve, and analyze the sequence data and impacted proteins and phenotypes in wee
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